Method for the simultaneous determination of blood group and platelet antigen genotypes
AssigneeCanadian Blood Services
InventorGregory A. Denomme
Life of this patent
Estimating the term…
Abstract
RBC and platelet (Plt) alloimmunization requires antigen-matched blood to avoid adverse transfusion reactions. Some blood collection facilities use unregulated Abs to reduce the cost of mass screening, and later confirm the phenotype with government approved reagents. Alternatively, RBC and Plt antigens can be screened by virtue of their associated single nucleotide polymorphisms (SNPs). We developed a multiplex PCR-oligonucleotide extension assay using the GenomeLab SNPStream platform to genotype blood for a plurality of blood group antigen-associated SNPs, including but not limited to: RhD (2), RhC/c, RhE/e, S/s, K/k, Kpa/b, Fya/b, FY0, Jka/b, Dia/b, and HPA-1a/b.
Claims11
-19. (canceled)
A probe having (i) at least one of the nucleotide sequence of nucleotide positions 1 to 20 of any one of SEQ ID NO:
- 25 to 35 and nucleotide position 1 to 18 of SEQ ID NO:
- 6 and (ii) the nucleotide sequence of nucleotide positions 21 to 45 of as set forth in SEQ ID NO:
- 25.
A method of detecting a RhD/RhCE blood group antigen in a sample, said method comprising:
- (a) providing genomic DNA from said sample;
- (b) submitting the genomic DNA of step (a) to a PCR amplification with at the first oligonucleotide as defined in claim 3 and the second oligonucleotide as defined in claim 3 to obtain at least one amplification product;
- and
- (c) analyzing the at least one amplification product of step (b) to detect the blood group antigen in the sample.
Dependencies are inferred from the claim text.