Complex sets of mirnas as non-invasive biomarkers for colon cancer
AssigneeComprehensive Biomarker Center GmbH
InventorsAndreas Keller, Markus Beier, Eckart Meese, Petra Leidinger, Anke Wendschlag
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Abstract
The present invention relates to non-invasive methods, kits and means for diagnosing and/or prognosing of colon cancer in a body fluid sample from a subject. Further, the present invention relates to set of polynucleotides or sets of primer pairs for detecting sets of miRNAs for diagnosing and/or prognosing of colon cancer in a body fluid sample from a subject. In addition, the present invention relates to sets of miRNAs for diagnosing and/or prognosing of colon cancer in a body fluid sample from a subject.
Claims14
A method for diagnosing and/or prognosing of colon cancer comprising the steps of:
- (i.) determining an expression profile of a set comprising at least two miRNAs representative for colon cancer in a blood sample from a subject, and
- (ii.) comparing said expression profile to a reference, wherein the comparison of said expression profile to said reference, allows for the diagnosis and/or prognosis of colon cancer,
- wherein the nucleotide sequences of the miRNAs comprised in the set are selected from the group consisting of SEQ ID NO:
- 1 to 588, a fragment thereof, and a sequence having at least 80% sequence identity thereto.
5-11. (canceled)
A kit for diagnosing and/or prognosing of colon cancer comprising
- (i.) means for determining an expression profile of a set comprising at least two miRNAs representative for colon cancer in a blood sample from a subject, and
- (ii.) at least one reference
- wherein the reference is determined from reference expression profiles of at least 2 control subjects with at least 2 clinical conditions, from which at least one is colon cancer.
13-17. (canceled)
A method for determining the status and/or the response of the immune system in a subject having or suspected of having colon cancer, comprising the steps of
- (i.) determining an expression profile of a set comprising at least two miRNAs representative for the status and/or the response of the immune system in a blood sample from a subject, and
- (ii.) comparing said expression profile to a reference, wherein the comparison of said expression profile to said reference allows for determining the status and/or the response of the immune system in said subject
- wherein the miRNAs comprised in the set are selected from the group consisting of SEQ ID NO:
- 1 588, a fragment thereof, and a sequence having at least 80% sequence identity thereto.
Dependencies are inferred from the claim text.