Application of reduced dyes in imaging

A compound having Formula I:

• wherein
• each R1 is an independently selected alkyl group that is additionally substituted with from 0 to 1 R14 and from 0 to 1 -L-Y—Z;
• wherein the alkyl is optionally interrupted by at least
• R1a is either hydrogen or deuterium;
• each R2a and R2b is a member independently selected from the group consisting of alkyl, alkenyl, hydroxyalkyl, alkoxyalkyl, aminoalkyl, amidoalkyl, alkylthioalkyl, carboxyalkyl, alkoxycarbonylalkyl, or sulfonatoalkyl;
• wherein a carbon of the member is additionally substituted with from 0 to 1 -L-Y—Z;
• each R3, R4, R5, and R6 is a member independently selected from the group consisting of hydrogen, alkyl, alkenyl, halo, hydroxyl, alkoxy, amino, cyano, carboxyl, alkoxycarbonyl, amido, sulfonato, alkoxyalkyl, carboxyalkyl, alkoxycarbonylalkyl, and sulfonatoalkyl;
• wherein a carbon of the member is additionally substituted with from 0 to 1 -L-Y—Z;
• R8, R9, R10, R11 and R12 are each a member independently selected from the group consisting of hydrogen, alkyl, alkenyl, halo, alkoxy, sulfonato, hydroxyl, amino, carboxyl, alkoxycarbonyl, cyano, amido, thioacetyl, and -L-Y—Z;
• wherein at least one member selected from the group consisting of R8, R9, and R10 is halo;
• each R13 is a member independently selected from the group consisting of hydroxyl, amino, carboxyl, and alkoxycarbonyl;
• each R14 is a member independently selected from the group consisting of alkyl, alkenyl, halo, hydroxyl, alkoxy, amino, amido, amidoalkyl, cyano, cyanoalkyl, carboxyl, alkoxycarbonyl, amido, sulfonato, sulfonatoalkyl, thioacetyl, thioacetylalkyl, alkoxycarbonylalkyl, and alkoxyalkyl;
• wherein the alkyl or alkenyl is additionally substituted with from 0 to 1 R13 and from 0 to 1 -L-Y—Z;
• each L is an optional member independently selected from the group consisting of a bond, a C1-C20 alkylene, and a C1-C20 alkenylene;
• wherein the alkylene or alkenylene is optionally interrupted by at least one heteroatom;
• each Y is an optional member independently selected from the group consisting of a bond, —O—, —S—, —NH—, —NHC(O)—, —C(O)NH—, —NR15—, —NR15C(O)—, —C(O)NR15—, —N(Z)—, —N(Z)C(O)—, and —C(O)N(Z)—;
• each Z is independently selected from the group consisting of -L-R13 and -L-R16;
• or alternatively, —Y—Z is a member selected from the group consisting of —N(Z)2, —N(Z)C(O)Z, and —C(O)N(Z)2, and the two Z groups may optionally be linked to form a cycloalkynyl group;
• each R15 is a member independently selected from the group consisting of alkyl and alkoxycarbonylalkyl;
• wherein the alkyl is optionally interrupted by at least one heteroatom;
• each R16 is independently a member selected from the group consisting of activated acyl, acrylamido, optionally substituted alkylsulfonate ester, azido, optionally substituted arylsulfonate ester, optionally substituted amino, aziridino, boronato, cycloalkynyl, cycloalkynylcarbonyl, diazo, formyl, glycidyl, halo, haloacetamidyl, haloalkyl, haloplatinato, halotriazino, hydrazinyl, imido ester, isocyanato, isothiocyanato, maleimidyl, mercapto, phosphoramidityl, a photoactivatable moiety, vinyl sulfonyl, alkynyl, cycloalkynyl, spirocycloalkynyl, a pegylated azido, a pegylated alkynyl, a pegylated cycloalkynyl, a pegylated spirocycloalkynyl, an o-diarylphosphino aryl ester, and an ortho substituted phosphine oxide aryl ester;
• and
• wherein said compound has a balanced charge.