Centrifugally-enhanced capture method and device
AssigneeNational Research Council of Canada
InventorsLiviu Clime, Xuyen Dai Hoa, Teodor Veres, Francois Normandin
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Abstract
In a centrifugal microfluidic device for conducting capture assays, a microfluidic platform rotates in a plane of rotation and has at least one capture surface for immobilizing a target particle of interest in the device. The capture surface oriented so that it is not parallel to the plane of rotation of the device and is positionally fixed in the device during operation of the device. The centrifugal force arising from rotation of the device forces the target particles against the capture surface. Capture efficiency is independent of the rate of flow of the fluid and independent of the rate of rotation of the microfluidic platform.
Claims22
A centrifugal microfluidic device for conducting capture assays, the device comprising a rotating microfluidic platform that rotates in a plane of rotation, the platform having at least one capture surface for immobilizing a target particle of interest in the device, the capture surface oriented so that it is not parallel to the plane of rotation of the device, the capture surface positionally fixed in the device during operation of the device, and centrifugal force arising from rotation of the device forces the target particles against the capture surface.
(canceled)
A method of capturing a target particle of interest for an assay in a centrifugal microfluidic device, the method comprising:
- introducing a fluid containing the target particle into a rotatable microfluidic platform of the microfluidic device;
- rotating the microfluidic platform in a plane of rotation to generate centrifugal force in the device;
- and, using the centrifugal force to direct flow of the fluid to a capture surface in the device thereby pushing the target particle against the capture surface to increase probability of the target particle interacting with the capture surface, wherein capture efficiency of the capture surface for the target particle is independent of rate of flow of the fluid and independent of rate of rotation of the microfluidic platform.
Dependencies are inferred from the claim text.