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US 2014/0212990 A1Published application
Detection assays employing magnetic nanoparticles
AssigneeBio-Rad Haifa Ltd.
InventorsShai NIMRI, Vered BRONNER
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Abstract
The present invention is directed to novel assays for detecting target molecules. The assays employ small size, detectably labeled, magnetic nanoparticles associated with a capture molecule. The detection assay is accelerated by applying magnetic field during the assay. The assays of the invention can be used to enhance the efficiency of the detection step in dot blot, Western blot and ELISA.
Claims24
An assay for detecting a target molecule comprising:
- a. Incubating a substrate potentially comprising at least one target molecule with detectably labeled magnetic nanoparticle (MNP) complexes, wherein said MNP complexes comprise MNP having a small diameter associated with a capture molecule capable of binding directly or indirectly to said target molecule;
- b. Applying magnetic field;
- and
- c. Subjecting the incubated substrate to a detection step;
- whereby the presence of a detection signal is indicative of the presence of the target molecule and the signal intensity is indicative of the amount of the target molecule.
2 more elements
An assay for detecting a target molecule comprising:
- a. Providing detectably labeled magnetic nanoparticle (MNP) complexes, wherein said MNP complexes comprise MNP associated with a secondary binding agent capable of binding a primary binding agent;
- b. Incubating said MNP complexes with a primary binding agent, thereby forming a second MNP complex comprising MNP, a secondary binding agent, and a primary binding agent;
- c. Incubating a substrate potentially comprising at least one target molecule with said second MNP complex;
- d. Applying magnetic field;
- and
- e. Subjecting the incubated sample to a detection reaction;
- whereby the presence of a detection signal is indicative of the presence of the target molecule and the signal intensity is indicative of the amount of the target molecule.
4 more elements
An assay system comprising a substrate potentially comprising at least one target molecule;
- at least one detectably labeled MNP complex, wherein said MNP complex comprises MNP having a small diameter associated with a capture molecule capable of binding directly or indirectly to said target molecule;
- and at least one magnet.
Dependencies are inferred from the claim text.