Signal Ratio in Assay Calibrators

A method of enhancing signal ratio between calibrators in an assay for an analyte, the method comprising:

• conducting the assay for the analyte with zero concentration of analyte in a first calibrator to determine a first signal level, wherein reagents employed in the assay comprise (i) an antibody reagent comprising an antibody having a thioether linkage between a hinge region of the antibody and a small molecule, (ii) a chemiluminescent particle reagent comprising an analyte analog, and (iii) a photosensitizer particle reagent comprising a small molecule-binding moiety;
• conducting the assay for the analyte with a second concentration of analyte in a second calibrator to determine a second signal level wherein the second concentration of analyte is greater than zero and wherein the reagents employed in the assay comprise the antibody reagent, the chemiluminescent particle reagent and the photosensitizer particle reagent;
• and
• determining the ratio of the first signal level to the second signal level.