Recombinant microorganisms comprising NADPH dependent enzymes and methods of production therefor
AssigneeLanzatech New Zealand Limited
InventorsAlexander Paul Mueller, Michael Koepke
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Abstract
The invention provides a recombinant carboxydotrophic Clostridia microorganism with increased overall utilization of NADPH relative to a parent microorganism. Further, the invention provides a method of producing a recombinant carboxydotrophic Clostridia microorganism which exhibits increased NADPH utilization relative to a parental microorganism. In particular, the invention relates to increasing the overall utilization of NADPH in a recombinant carboxydotrophic Clostridia microorganism in order to increase the production of at least one fermentation product by the microorganism.
Claims27
A recombinant carboxydotrophic Clostridia microorganism adapted to express at least one exogenous NADPH-dependent enzymes or adapted to over-express at least one endogenous NADPH-dependent enzymes, the enzymes selected such that when the exogenous enzyme is expressed or the endogenous enzyme is overexpressed, the overall utilization of NADPH by the microorganism is increased relative to a parental microorganism.
A method of producing a recombinant carboxydotrophic Clostridia microorganism which exhibits increased NADPH utilization relative to a parental microorganism, the method comprising:
- a. selecting at least one exogenous or endogenous NADPH-dependent enzyme;
- b. transforming a parental microorganism to produce a recombinant microorganism which is adapted to express the at least one NADPH-dependent exogenous enzyme or overexpress the at least one NADPH-dependent endogenous enzyme.
Dependencies are inferred from the claim text.