Compositions and methods for bioculture of wasabia japonica
OwnerJackie R Heinricher, Randall Burr +1inventors
InventorsJackie R Heinricher, Randall Burr, Jerrin Victor
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Abstract
The present invention provides media, kits, systems, and methods for achieving large scale wasabi production within a short time via bioculture. The present invention for wasabi production results in shorter tuber development phase and higher yield.
Claims29
A set of media for producing wasabi plants or plant parts wherein the set of media comprises:
- (1) one or more initiation medium;
- (2) one or more multiplication medium;
- and
- (3) one or more rooting medium,
- wherein the initiation medium and/or the multiplication medium comprises 6-Benzylaminopurine (BAP) or any functional derivative, and indole-3-acetic acid (IAA) or any functional derivative thereof, and the rooting medium comprises IAA and does not comprise any cytokinin.
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A method for producing wasabi plant in vitro comprising:
- (a) obtaining a wasabi explant;
- (b) initiating shoot from the explant obtained in step (a) on an initiation medium;
- (c) multiplying the shoot initiated from step (b) on a multiplication medium;
- and
- (d) transferring the multiplied shoots of step (c) on a rooting medium to produce a wasabi plant with root;
- wherein the wasabi explant is obtained from a rhizome shoot tip, and wherein the initiation medium and/or the multiplication medium comprises 6-Benzylaminopurine (BAP) or any functional derivative, and indole-3-acetic acid (IAA) or any functional derivative thereof, and the rooting medium comprises IAA and does not comprise any cytokinin.
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The method of claim 3, wherein every 1 gram of wasabi tissue is supported by about 100 ml medium.
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Dependencies are inferred from the claim text.