Mast Cell Culture and Methods to Produce the Culture
AssigneeApollonian Biosystems Limited
InventorsSee-Ying TAM, Yee San Issan TAM, Hang Yung Alaster LAU
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Abstract
The present invention relates to mast cell cultures that are derived from hematopoietic progenitors and the use thereof. The invention describes a method for generating in-vitro cultures of human mast cells with functional phenotype of connective tissue-type mast cells. By monitoring the levels of chemokines released into the medium, such mast cell cultures can be used as a cell-based assay to assess regulation of mast cell functions and pharmacological activities of tryptase inhibitors.
Claims17
A method to make a mast cell culture comprising the steps of (a) treating CD34+ progenitor cells under hypoxic condition for 1 week in a medium comprising SCF, IL-6 and IL-3 to generate an initial-stage cell culture;
- (b) treating the initial-stage cell culture under hypoxic condition for about 0.5-2.5 weeks in a medium comprising SCF, IL-6 and IL-9 to form an intermediate stage cell culture;
- (c) treating the intermediate-stage cell culture under normoxic condition for 2.5-3.5 weeks in a medium comprising SCF and IL-6 to generate an immature mast cell culture.
Dependencies are inferred from the claim text.