Method and kit for culturing stem cells
AssigneeChina Medical University
InventorsSHIH-PING LIU, SHINN-ZONG LIN, HORNG-JYH HARN, YING-JIUN CHIEN, CHIEN-YU HSU, CHENG-HSUAN CHA
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Abstract
A method is provided, including adding a phthalide to a stem cell medium to provide a phthalide-containing medium, and then culturing a stem cell using the phthalide-containing medium. The use of a phthalide in a medium for culturing stem cells optionally maintains the pluripotency of stem cells. The phthalide also enhances the generation efficiency of induced pluripotent stem cells to decrease the culture cost.
Claims21
A method for culturing a stem cell, comprising the following steps:
- (A) providing a stem cell medium;
- (B) adding a phthalide into the stem cell medium to provide a phthalide-containing medium;
- and
- (C) using the phthalide-containing medium to culture a stem cell.
A kit for culturing a stem cell, consisting of (1) a stem cell medium;
- (2) n-butylidenephthalide (BP);
- and (3) and optional solvent n-butylidenephthalide (BP).
-17. (canceled)
(canceled)
A kit for culturing a stem cell, comprising (1) a stem cell medium;
- and (2) a purified n-butylidenephthalide (BP).
Dependencies are inferred from the claim text.