Cytochrome p450s and uses thereof

A host cell, comprising nucleic acid encoding an isoprenoid synthase and nucleic acid encoding cytochrome P450 polypeptides, wherein:

• the nucleic acid encoding the synthase and the nucleic acid encoding the P450 polypeptides are heterologous to the host cell;
• the synthase catalyzes production of an isoprenoid compound;
• the P450 polypeptides catalyze hydroxylation, oxidation, demethylation or methylation of an isoprenoid compound;
• at least one of the P450 polypeptide(s) catalyzes the hydroxylation, oxidation, demethylation or methylation of an isoprenoid compound whose production is catalyzed by the synthase;
• and
• the host cell produces a compound not normally produced by the host cell in the absence of the P450 polypeptides.

A method for producing a host cell comprising an isoprenoid compound, comprising:

• a) providing a host cell that comprises heterologous nucleic acid encoding an isoprenoid synthase and heterologous nucleic acid encoding one or more protein(s) comprising a cytochrome P450 polypeptide, wherein:
• the isoprenoid synthase catalyzes production of an isoprenoid compound;
• and
• a cytochrome P450 polypeptide(s) catalyzes hydroxylation, oxidation, demethylation or methylation of an isoprenoid compound;
• b) culturing the host cell under conditions suitable for expressing the isoprenoid synthase and the cytochrome P450 polypeptide(s) and under conditions for producing an isoprenoid compound not normally produced by the host cell to produce an isoprenoid compound not normally produced by the host cell, wherein the synthase and the cytochrome P450 polypeptide(s) together catalyze the formation of an isoprenoid compound not normally produced by the cell;
• and
• c) isolating the host cell that comprises an isoprenoid compound not normally produced by the cell.

A method for producing an isoprenoid compound, comprising:

• a) providing a host cell that expresses heterologous nucleic acid encoding a first recombinant protein comprising an isoprenoid synthase, and a second recombinant protein comprising a cytochrome P450 polypeptide, wherein:
• the isoprenoid synthase catalyzes production of an isoprenoid compound;
• and
• the cytochrome P450 polypeptide catalyzes hydroxylation, oxidation, demethylation or methylation of the isoprenoid compound produced by the synthase to produce an isoprenoid compound not normally produced by the host cell nor whose production is catalyzed by the synthase;
• b) culturing the host cell under conditions suitable for expressing the first recombinant protein comprising an isoprenoid synthase and the second recombinant protein comprising the cytochrome P450 polypeptide and under conditions for producing the isoprenoid compound not normally produced by the host cell, wherein the first and second recombinant protein together catalyze the formation of an isoprenoid compound;
• and
• c) isolating an isoprenoid compound not normally produced by the host cell.

A method for producing a host cell comprising an isoprenoid compound, comprising:

• a) providing a host cell that comprises heterologous nucleic acid encoding one or more protein(s) comprising a cytochrome P450 polypeptide, wherein:
• a cytochrome P450 polypeptide(s) catalyzes hydroxylation, oxidation, demethylation or methylation of an isoprenoid;
• and
• b) culturing the host cell under conditions suitable for expressing the cytochrome P450 polypeptide(s) and under conditions for producing an isoprenoid compound not normally produced by the host cell to produce an isoprenoid compound not normally produced by the host cell, wherein the cytochrome P450 polypeptide(s) catalyze the formation of an isoprenoid compound in the host cell, wherein the isoprenoid compound is not normally produced by the cell.