Over-expression of a putative oxidoreductase (ucpa) for increasing furfural or 5-hydroxymethylfurfural tolerance
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsXuan Wang, Elliot N. Miller, Lorraine P. Yomano, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Abstract
The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.
Claims15
1-54. (canceled)
An isolated bacterial, fungal or yeast cell having increased UcpA activity as compared to a reference bacterial, fungal or yeast cell, wherein said bacterial, fungal or yeast cell having increased UcpA activity exhibits increased tolerance to furfural and/or 5-hydroxymethylfurfural (5-HMF).
A method of increasing furfural and/or 5-hydroxymethylfurfural (5-HMF) resistance in a bacterial, fungal or yeast cell comprising increasing UcpA activity in said bacterial, fungal or yeast cell, as compared to a reference bacterial, fungal or yeast cell, wherein said bacterial, fungal or yeast cell having increased UcpA activity increases resistance of said bacterial, fungal or yeast cell to furfural and/or 5-hydroxymethylfurfural (5-HMF).
Dependencies are inferred from the claim text.