Back to results
US 2014/0212929 A1Published application
Dna polymerases with increased 3'-mismatch discrimination
AssigneeRoche Molecular Systems, Inc.
InventorsFred Reichert, Keith Bauer, Thomas W. Myers
Life of this patent
Estimating the term…
Abstract
Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
Claims21
A DNA polymerase having increased 3′-mismatch discrimination activity compared with a control DNA polymerase, wherein the DNA polymerase comprises a motif in the polymerase domain comprising
- P-N-L-Q-N-X1-P-X2-X3-X4-X5-X6-G, wherein:
- X1 is I or L;
- X2 is I, V or T;
- X3 is any amino acid other than R or K;
- X4 is T or S;
- X5 is P or E;
- and
- X6 is L or E (SEQ ID NO:9).
5 more elements
Dependencies are inferred from the claim text.