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US 2014/0212924 A1Published application
Human recombinant growth and differentiaton factor-5 (rhgdf-5)
AssigneeWarsaw Orthopedic, Inc.
InventorsDavid S. Scher, Roger E. Harrington
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Abstract
Expression vector systems are provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided are transformed host cells that were engineered to produce and express high levels of rhGDF-5 protein. Methods for production and high expression of rhGDF-5 protein are disclosed herein. The methods of enhancing production and protein expression of rhGDF-5 protein as disclosed are cost-effective, time-saving and are of manufacturing quality.
Claims20
An expression vector comprising:
- a T5 promoter operably linked to a polynucleotide sequence that encodes a GDF-5 protein.
A method for producing an rhGDF-5 (rhGDF-5) protein, the method comprising:
- providing a prokaryotic host cell comprising an expression vector which comprises a polynucleotide encoding an rhGDF-5 protein under the control of a T5 or Trc promoter;
- and
- cultivating the prokaryotic host cell under suitable conditions so as to induce or promote the expression of the polynucleotide in the expression vector and
- recovering the rhGDF-5 protein.
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Dependencies are inferred from the claim text.