Two plasmid mammalian expression system

A two plasmid system for producing recombinant proteins using RNA Dependent RNA Polymerase of non-segmented negative strand RNA viruses comprising:

• a. one cloning plasmid comprising an easily manipulatable replicon at two multiple cloning, and
• b. one helper plasmid comprising N, P, L genes expressing N, P, L proteins respectively;
• without the help of a replicating helper vaccinia virus or exogenous RNA polymerase.

A two plasmid system for producing infectious non-segmented negative strand RNA viruses using

• a. one virus cloning plasmid comprising a cDNA encoding entire viral genomic RNA for insertion of expressible DNA fragment;
• b. one helper plasmid comprising N, P, L genes expressing N, P, L proteins respectively
• without the help of a replicating helper vaccinia virus or exogenous RNA polymerase.

A two plasmid system for producing recombinant proteins using Measles virus RNA Dependent RNA Polymerase of non-segmented negative strand RNA viruses comprising:

• a. one cloning plasmid comprising an easily manipulatable measles virus replicon at two multiple cloning sites for insertion of expressible DNA fragment;
• b. one helper plasmid comprising measles virus N, P, genes expressing measles virus N, P, L proteins respectively;
• without the help of a replicating helper vaccinia virus or exogenous RNA polymerase.

A two plasmid system for producing infectious measles virus comprising:

• a. one virus cloning plasmid comprising a cDNA encoding entire measles virus genomic RNA for insertion of expressible DNA fragment;
• b. one helper plasmid comprising measles virus N, P, L genes expressing measles virus N, P, L proteins respectively;
• without the help of a replicating helper vaccinia virus or exogenous RNA polymerase.