Cell cultures and methods of human recombinant growth and differentiaton factor-5 (rhgdf-5)
AssigneeWarsaw Orthopedic, Inc.
InventorsDavid S. Scher, Roger E. Harrington
Life of this patent
Estimating the term…
Abstract
A cell cuture media for plasmid production is provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided herein is a method of making a cell culture media for plasmid production and a method for growing a plasmid vector. The methods of enhancing production and protein expression of rhGDF-5 protein as disclosed are cost-effective, time-saving and are of manufacturing quality.
Claims20
A cell culture media for growing a cell that expresses rhGDF-5 protein, the cell culture media supporting growth of a cell that comprises an expression vector having a polynucleotide encoding an rhGDF-5 protein under the control of a T5 or Trc promoter.
A method of making a cell culture media for growing a cell that expresses rhGDF-5 protein, the method comprising providing the cell culture media supporting growth of the cell that comprises an expression vector having a polynucleotide encoding an rhGDF-5 protein under the control of a T5 or Trc promoter.
A method of growing a cell that expresses rhGDF-5 protein, the method comprising growing in a cell culture media a cell that comprises an expression vector having a polynucleotide encoding an rhGDF-5 protein under the control of a T5 or Trc promoter.
Dependencies are inferred from the claim text.