Mutant gamma-glutamyltransferase, and a method for producing gamma-glutamylvalylglycine or a salt thereof
AssigneeAjinomoto Co., Inc.
InventorsHiroyuki NOZAKI, Isao Abe, Jun Takakura, Rie Takeshita, Hideyuki Suzuki, Shunichi Suzuki
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Abstract
A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation.
Claims16
A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein:
- the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has the amino acid sequence of the positions 391 to 580 of SEQ ID NO:
- 2 or the amino acid sequence having a homology of 90% or more to the foregoing amino acid sequence, and has a mutation for one or more residues corresponding to one or more residues selected from the following residues in the amino acid sequence of SEQ ID NO:
- 2:
- N411, T413, Q430, P441, V443, Y444, L446, A453, D472, G484, S498, Q542, D561, S572.
A mutant γ-glutamyltransferase consisting of the following large subunit and small subunit:
- (A) a large subunit which has the amino acid sequence of the positions 26 to 390 of SEQ ID NO:
- 2 or the amino acid sequence including substitutions, deletions, insertions, additions, or inversions of one or several amino acid residues, and able to form a complex having the γ-glutamyltransferase activity with any one of the following small subunit;
- (B) a small subunit which has the amino acid sequence of the positions 391 to 580 of SEQ ID NO:
- 2 or the amino acid sequence including substitutions, deletions, insertions, additions, or inversions of one or several amino acid residues, has any one of the following mutations, and able to form a complex having the γ-glutamyltransferase activity with the above large subunit:
- Y444D, Y444E, (T413A+Y444E), (T413H+Y444E), (T413N+Y444E), (Q430N+Y444E), (Q430N+Y444D), (Q430N+Y444N), (P441A+Y444E), (V443A+Y444E), (V443E+Y444E), (V443G+Y444E), (V443L+Y444E), (V443N+Y444E), (V443Q+Y444E), (Y444E+L446A), (Y444E+A453S), (Y444E+D472I), (Y444E+G484A), (Y444E+G484S), (Y444E+S498C), (Y444E+Q542H), (Y444E+D561N), (T413N+V443A+Y444E), (T413N+Y444E+A453S), (T413N+Y444E+S498C), (T413N+Y444E+Q542H), (G484S+Y444E+V443A), (G484S+Y444E+Q542H), (Q430N+Y444E+T413N), (T413H+Y444E+G484S), (T413N+Y444E+G484S), (T413N+Y444E+G484S+V443A), (T413N+Y444E+G484S+A453S), (T413N+Y444E+G484S+Q542H), (T413N+Y444E+G484S+S572K), (T413N+Y444E+G484S+Q430N), (T413N+Y444E+G484E+S498C).
Dependencies are inferred from the claim text.