US 2014/0212919 A1 · PROTEIN SYNTHESIS KIT, AND METHOD FOR EXPRESSING AND EXTRACTING PROTEINS USING AUTOMATIC EXTRACTION EQUIPMENT

Abstract

Provided is a method of protein synthesis. The method of protein synthesis according to the present invention uses an automatic biological material purification apparatus including: a well plate kit; a heating part; and a magnetic field applying part, such that a plurality of target proteins may be more quickly and simply obtained as compared to target proteins obtained by using the existing method for expressing/purifying proteins through conventional cell culture, and a reproducible synthesis efficiency on the same proteins may be obtained due to no deviation between reaction wells.

Claims19

Dependent claims

A method of protein synthesis, the method comprising:

(1) preparing a template for cell-free protein synthesis;
(2) adding the template to a cell-free protein expression solution to express proteins;
(3) adding magnetic particles coupled with an affinity tag to the expressed protein to attach a target protein to the magnetic particles;
and
(4) separating the attached target protein from the magnetic particles,
wherein expression and purification of the target protein are simultaneously performed in an automatic system using an automatic biological material purification apparatus including:
a heating part;
and a magnetic field applying part.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/238,908Filed 23 Aug 2012

Priority

KR 10-2011-008582426 Aug 2011

Published

31 Jul 2014

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12P 21/00
Preparation of peptides or proteins
B01J 19/0046
Chemical, physical or physico-chemical processes in general; Their relevant apparatus: sequential or parallel reactions, e.g. for the synthesis of…
C07K 1/22
By chromatography: affinity chromatography or related techniques based upon selective absorption processes
C12P 21/02
Preparation of peptides or proteins: having a known sequence of two or more amino acids, e.g. glutathione

Protein synthesis kit, and method for expressing and extracting proteins using automatic extraction equipment

Life of this patent

Protein synthesis kit, and method for expressing and extracting proteins using automatic extraction equipment

Life of this patent

Abstract

Claims19