Method for preparing human neoplastically transformed cells
AssigneeTRDIGM & Co., Ltd.
InventorKatsutomo HAMADA
Life of this patent
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Abstract
A method for preparing neoplastically transformed cells from human-derived cells, including the step of introducing human telomerase catalytic subunit (hTERT) gene, SV40 small T antigen (SV40ST) gene, and an antisense oligonucleotide derived from human 28S rRNA into the human-derived cells. The method for preparing neoplastically transformed cells from human-derived cells can be utilized when a variety of human normal cells are induced to be neoplastically transformed in order to elucidate cancer onset mechanisms, so that the method can be effectively utilized in search of target molecules for a new medicament.
Claims18
1-7. (canceled)
A method for screening an anticancer agent, comprising:
- neoplastically transforming at least two isolated human cells by introducing a human telomerase catalytic subunit (hTERT) gene, an SV40 small T antigen (SV40ST) gene, and an antisense oligonucleotide isolated from a human 28S ribosomal RNA (rRNA) into the isolated human cells, to obtain at least two neoplastically transformed cells;
- culturing a first neoplastically transformed cell in the presence of a candidate compound;
- culturing a second neoplastically transformed cell in the absence of the candidate compound;
- measuring a degree of inhibition of the first neoplastically transformed cell and a degree of inhibition of the second neoplastically transformed cell;
- and
- judging that the candidate compound has a high possibility of having an action as an anticancer agent when the degree of inhibition of the first neoplastically transformed cell is greater than the degree of inhibition of the second neoplastically transformed cell.
(canceled)
Dependencies are inferred from the claim text.