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US 2014/0212907 A1Published application
Methods and Kits for Measuring Toxicity and Oxidative Stress in Live Cells
AssigneeLankenau Institute for Medical Research
InventorIraimoudi S. Ayene
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Abstract
Compositions and methods for the measuring toxicity, antioxidant capacity, and oxidative stress are provided.
Claims23
A method for measuring oxidative stress in a cell, said method comprising:
- a) contacting said cells with hydroxyethyldisulfide (HEDS);
- and
- b) determining the amount of extracellular mercaptoethanol;
- wherein the amount of extracellular mercaptoethanol is inversely proportional to the oxidative stress of said cell.
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A method for screening for a modulator of antioxidant activity involved in cell survival, said method comprising:
- a) contacting said antioxidant with a compound;
- b) contacting the mixture of step a) with hydroxyethyldisulfide (HEDS);
- and
- c) determining the amount of mercaptoethanol in the mixture of step b);
- wherein a modulation in the amount of mercaptoethanol compared to amount of mercaptoethanol in the absence of said compound indicates that said compound is a modulator of antioxidant activity.
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A kit for measuring glutathione recycling and cell survival, said kit comprising:
- a) 5,5-dithiobis 2-nitrobenzoic acid (DTNB);
- b) hydroxyethyldisulfide (HEDS);
- and
- c) sulfosalicyclic acid buffer (SSA).
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Dependencies are inferred from the claim text.