Screening Method
AssigneesGuy's and St. Thomas' NHS Foundation Trust, King's College London
InventorsCharles Turner, Raymond Neil Dalton, Yvonne Anne Daniel
Life of this patent
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Abstract
A method for screening for variant peptides uses mass spectrometry (MS). A system and a kit may be used for performing the method. Proteins in a sample are digested to form a defined series of peptides. The defined series of peptides are ionized. The ionized species are subjected to collision induced dissociation. Species of known mass/charge ratio are detected to confirm the presence of the protein variant in the original sample.
Claims17
A method for detecting a known protein variant in a sample, wherein the sequence of the protein variant to be detected is known, the method comprising:
- (i) digesting the protein to produce a defined series of peptides;
- (ii) ionising the peptides and selecting by mass spectrometry an ionised species of known mass/charge ratio indicative of the protein variant;
- and
- (iii) subjecting the selected ionised species to collision induced dissociation and measuring a single derived ionised species of known mass/charge ratio that confirms the presence of the protein variant in the sample.
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Dependencies are inferred from the claim text.