Novel Method for Detection of Beta-D-Glucuronidase
AssigneeBiosynth AG
InventorsUrs Spitz, Lukas Wick, Günter Schabert
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Abstract
A method for detection of beta-D-glucuronidase activity in a sample that comprises the steps of (i) providing a glucuronic acid ester compound of the general formula (I) wherein R1 is a C1-4 alkyl group, OR2 is a dye moiety, which is liberated after cleavage of the glycosidic bond; (ii) contacting said glucuronic acid ester compound with a material of said sample exhibiting hydrolytic activity towards glucuronic acid esters, thereby removing R1 and thus forming a sample containing an indicator compound suitable for the detection and/or measurement of beta-D-glucuronidase activity, and (iii) using said indicator to perform an assay requiring detection or measurement of beta-D-glucuronidase activity.
Claims22
A method for detection of beta-D-glucuronidase activity in a sample, comprising the steps of
- (i) providing a glucuronic acid ester compound of the general formula
- wherein
- R1 is a C1-4 alkyl group,
- OR2 is a dye moiety, which is liberated after cleavage of the glycosidic bond;
- (ii) contacting said glucuronic acid ester compound with a material contained in said sample, said material exhibiting hydrolytic activity towards glucuronic acid esters, thereby removing R1 and thus forming a sample containing an indicator compound suitable for the detection and/or measurement of beta-D-glucuronidase activity, and
- (iii) using said indicator to perform an assay requiring detection or measurement of beta-D-glucuronidase activity in said sample.
Dependencies are inferred from the claim text.