US 2014/0212879 A1 · METHOD FOR DETECTING TOXIN-PRODUCING CLOSTRIDIUM DIFFICILE

Abstract

Provided are an oligonucleotide which realizes specific and highly sensitive detection of toxigenic C. difficile, and a method for detecting toxigenic C. difficile, the method employing the oligonucleotide. 1) A primer pair containing an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 1, and an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 2; or a primer pair having complementary sequences to the nucleotide sequences. 2) A primer pair containing an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 4, and an oligonucleotide having a nucleotide sequence represented by SEQ ID NO: 5; or a primer pair having complementary sequences to the nucleotide sequences.

Claims20

Dependent claims

A primer pair comprising:

(i) an oligonucleotide comprising a nucleotide sequence represented by SEQ ID NO:
1, and an oligonucleotide comprising a nucleotide sequence represented by SEQ ID NO:
2;
or
(ii) an oligonucleotide comprising a nucleotide sequence that is complementary to SEQ ID NO:
1, and an oligonucleotide comprising a nucleotide sequence that is complementary to SEQ ID NO:
2.

4 more elements

A primer pair comprising:

(i) an oligonucleotide comprising a nucleotide sequence represented by SEQ ID NO:
4, and an oligonucleotide comprising a nucleotide sequence represented by SEQ ID NO:
5;
or
(ii) an oligonucleotide comprising a nucleotide sequence that is complementary to SEQ ID NO:
4, and an oligonucleotide comprising a nucleotide sequence that is complementary to SEQ ID NO:
5.

4 more elements

Dependencies are inferred from the claim text.

Method for detecting toxin-producing clostridium difficile

Life of this patent

Method for detecting toxin-producing clostridium difficile

Life of this patent

Abstract

Claims20