Maize linkage drag and genome analysis process
AssigneeAgrigenetics, Inc.
InventorsLindsay R. Manley, Joseph W. Spinks, Trisha Borowicz, Roanne Pangborn
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Abstract
This invention relates to a method for improving a maize linkage drag and genome analysis process. Some embodiments produce a significant cost and time reduction in plant breeding projects. Particular embodiments concern a method to determine one amount of linkage drag flanking a desired, introgressed trait and to determine a recurrent parent percentage performed at a same stage of plant growth. This disclosure also concerns selecting plants containing the desired, introgressed trait based on results of genome analysis.
Claims27
A method for analyzing plants which contain a desired, introgressed trait, the method comprising the steps of:
- analyzing a genome of a plant containing the desired, introgressed trait by (i) determining at least one amount of linkage drag flanking the desired trait on a chromosome containing the desired trait in the genome of the plant;
- and, (ii) determining a recurrent parent percentage present on all chromosomes in the genome of the plant, wherein both the determination of the linkage drag and the determination of recurrent parent percentage are performed at a same stage of plant growth;
- and selecting plants containing the desired, introgressed trait based on results of genome analysis.
A method of genome analysis comprising screening a genome by linkage drag genome analysis (LDGA) genotyping, wherein only one round of PCR is performed.
A method of genome analysis comprising delivering linkage drag genome analysis (LDGA) genotyping results solely by flowering.
Dependencies are inferred from the claim text.