Ceramide anionic liposome compositions

A method of producing a pharmaceutical composition, comprising:

• providing a lipid mixture comprising at least one pegylated neutral lipid, wherein the total amount of pegylated neutral lipid is an amount in the range of 5-20 Molar percent, inclusive, and wherein at least half of the amount of included pegylated neutral lipid is N-Octanoyl-Sphingosine-1-succinyl(methoxy(polyethylene)glycol)750 (PEG(750) C8);
• at least one anionic lipid, wherein the total amount of anionic lipid is an amount in the range of 5-15 Molar percent, inclusive;
• one or more ceramides selected from C4 ceramide, C6 ceramide, C8 ceramide, C10 ceramide, C12 ceramide, C14 ceramide, C16 ceramide and C18 ceramide, in an amount in the range of 1-40 Molar percent, inclusive;
• and cationic or neutral lipids, with the proviso that the resulting lipid mixture has a net negative charge at physiological pH;
• sonicating the lipid mixture in the presence of an amount of a antineoplastic chemotherapeutic at a temperature in the range of 55-75 degrees Celsius, inclusive, to produce a sonicated mixture;
• and
• passing the sonicated mixture through a filter having pores of a desired size to produce liposomes having the desired size, at a temperature in the range of 67-75 degrees Celsius, producing a population of ceramide anionic liposomes, wherein the population comprises greater than 10% of the amount of the antineoplastic chemotherapeutic.