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AssigneeCellseed Inc.
InventorsMasato KANZAKI, Masayuki YAMATO, Tinatsu KOHNO, Hidekazu SEKINE, Akihiko KIKUCHI, Teruo OKANO, Takamasa ONUKI
Estimating the term…
The problem to be solved by the present invention is to provide a cultured cell sheet having a superior tissue adhesiveness and an excellent flexibility. The problems can be solved by producing the cultured cell sheet in which cells are cultured in the presence of a surfactant protein or a cross-linking inhibitor on a surface of a cell culture support that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; and detaching the cultured cell sheet.
1-22. (canceled)
A method for preparing a cultured cell sheet for use in treatment of suppressing air leakage, blood leakage, or bodily fluid leakage from a surface layer of an organ, which has tissue adhesiveness and flexibility, the method comprising obtaining a cultured cell sheet by culturing cells including any one type or combination of two or more types of cells selected from the group consisting of fibroblasts, cells of alveolar tissue, cells of myocardial tissue, cells of hepatic tissue, cells of vascular tissue, mesenchymal stem cells, and adipose derived cells, on a cell culture support having a surface coated with a temperature responsive polymer having an upper or lower critical solution temperature of 0° C. to 80° C. in water, in the range of 0.5 μg/cm2 to 5.0 μg/cm2;
A method of treatment for suppressing air leakage, blood leakage, or bodily fluid leakage from a surface layer of an organ;
Dependencies are inferred from the claim text.