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AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsFelicito Guerrero, Adalberto A. Perez de Leon
Estimating the term…
Two antigenic and immunogenic proteins of the cattle tick, Rhipicephalus microplus, and the genes encoding these proteins, are effective for eliciting a protective immune response that controls and prevents infestations of bovines and other livestock by the tick. The proteins isolated from the cattle tick include an aquaporin protein and a TC5777 gut membrane protein. Each of the proteins elicit an immunoprotective response in livestock to the cattle tick, and can be formulated and administered as vaccines. Alternatively, the isolated DNA sequences which encode these proteins can be incorporated into nucleic acid constructs which could be utilized as DNA vaccines. The nucleic acid constructs can also be used for the transformation of cells and the production of recombinant proteins. Induction of the protective immune response controls and prevents infestations of the treated animals with the tick, thereby protecting them against tick-borne pathogen transmission.
A nucleic acid construct comprising a nucleic acid sequence encoding an immunogenic protein of the cattle tick, Rhipicephalus microplus, operatively linked to one or more expression control sequences, wherein said nucleic acid sequence comprises a nucleic acid sequence encoding aquaporin protein, a nucleic acid sequence encoding TC5777 gut membrane protein or combinations thereof.
The nucleic acid construct of claim i wherein said nucleic acid sequence encodes said aquaporin protein, and said aquaporin protein comprises amino acids 3-198 of SEQ ID NO:1.
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding an immunogenic aquaporin protein of the cattle tick, Rhipicephalus microplus.
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding an immunogenic TC5777 gut membrane protein of the cattle tick, Rhipicephalus microplus.
30-32. (canceled)
A composition comprising TC5777 gut membrane protein of the cattle tick, Rhipicephalus microplus, and a pharmaceutically acceptable carrier, wherein said TC5777 is in an amount effective to stimulate an immune response in bovine to said cattle tick, and wherein said TC5777 is selected from the group consisting of SEQ ID NO:
34-37. (canceled)
39-41. (canceled)
Dependencies are inferred from the claim text.