Polypeptide-vaccines for broad protection against hypervirulent meningococcal lineages
AssigneeNovartis AG
InventorMariagrazia PIZZA
Life of this patent
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Abstract
A small number of defined antigens can provide broad protection against meningococcal infection, and the invention provides a composition which, after administration to a subject, is able to induce an antibody response in that subject, wherein the antibody response is bactericidal against two or three of hypervirulent lineages A4, ET 5 and lineage 3 of N. meningitidis serogroup B. Rather than consisting of a single antigen, the composition comprises a mixture of 10 or fewer purified antigens, and should not include complex or undefined mixtures of antigens such as outer membrane vesicles. Five protein antigens are used in particular: (1) a ‘NadA’ protein; (2) a ‘741’ protein; (3) a ‘936’ protein; (4) a ‘953’ protein; and (5) a ‘287’ protein.
Claims30
A composition which, after administration to a subject, is able to induce an antibody response in that subject, wherein the antibody response is bactericidal against two or more of hypervirulent lineages A4, ET-5 and lineage 3 of N. meningitidis serogroup B.
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A polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NOs:
- 1 to 8.
A process for purifying soluble NadA from a culture medium, comprising the steps of:
- concentration and diafiltration against a buffer by ultrafiltration;
- anionic column chromatography;
- hydrophobic column chromatography;
- hydroxylapatite ceramic column chromatography;
- diafiltration against a buffer;
- and filter sterilisation.
A process for purifying a 936-ΔG741 hybrid protein from a bacterium, comprising the steps of:
- homogenisation;
- centrifugation;
- cationic column chromatography;
- anionic column chromatography;
- hydrophobic column chromatography;
- diafiltration against a buffer;
- and filter sterilisation.
Dependencies are inferred from the claim text.