Anti-EPHA2 Antibody
AssigneeDaiichi Sankyo Company, Limited
InventorsJun Hasegawa, Toshiaki Ohtsuka, Atsushi Urano, Junko Yamaguchi, Toshinori Agatsuma, Kaori Nakahara, Takeshi Takizawa
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Abstract
The present invention provides an antibody which recognizes an epitope recognized by an antibody produced by a hybridoma SH348-1 (FERM BP-10836) or a hybridoma SH357-1 (FERM BP-10837), an antibody produced by the hybridoma SH348-1 or the hybridoma SH357-1, an antibody obtained by humanizing the antibody produced by the hybridoma SH348-1 or the hybridoma SH357-1, a pharmaceutical agent comprising the antibody as an active ingredient, etc.
Claims51
An isolated antibody which binds specifically to EPHA2 and recognizes an epitope recognized by an antibody produced by the hybridoma SH348-1 (FERM BP-10836).
An isolated antibody which binds specifically to EPHA2 and recognizes an epitope recognized by an antibody produced by the hybridoma SH357-1 (FERM BP-10837).
An antibody produced by the hybridoma SH348-1 (FERM BP-10836).
An isolated antibody which binds specifically to EPHA2 consisting of the following 1) and 2):
- 1) a heavy chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 1 to 119 of SEQ ID NO:
- 35 in the sequence listing or a heavy chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 1 to 119 of SEQ ID NO:
- 39 in the sequence listing;
- and
- 2) a light chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 1 to 112 of SEQ ID NO:
- 37 in the sequence listing or a light chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 1 to 112 of SEQ ID NO:
- 41 in the sequence listing.
An isolated antibody which binds specifically to EPHA2 consisting of a heavy chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 1 to 119 of SEQ ID NO:
- 35 in the sequence listing and a light chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 1 to 112 of SEQ ID NO:
- 37 in the sequence listing.
An isolated antibody which binds specifically to EPHA2 consisting of the following 1) and 2):
- 1) a heavy chain polypeptide comprising the amino acid sequence represented by SEQ ID NO:
- 35 in the sequence listing or a heavy chain polypeptide comprising the amino acid sequence represented by SEQ ID NO:
- 39 in the sequence listing;
- and
- 2) a light chain polypeptide comprising the amino acid sequence represented by SEQ ID NO:
- 37 in the sequence listing or a light chain polypeptide comprising the amino acid sequence represented by SEQ ID NO:
- 41 in the sequence listing.
An isolated antibody which specifically binds to EPHA2 consisting of a heavy chain polypeptide comprising the amino acid sequence represented by SEQ ID NO:
- 35 in the sequence listing and a light chain polypeptide comprising the amino acid sequence represented by SEQ ID NO:
- 37 in the sequence listing.
An isolated antibody which binds specifically to EPHA2 consisting of the following 1) and 2):
- 1) a heavy chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 468 of SEQ ID NO:
- 107 in the sequence listing or a heavy chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 468 of SEQ ID NO:
- 115 in the sequence listing;
- and
- 2) a light chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 239 of SEQ ID NO:
- 91 in the sequence listing or a light chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 239 of SEQ ID NO:
- 99 in the sequence listing.
An isolated antibody which binds specifically to EPHA2 consisting of a heavy chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 468 of SEQ ID NO:
- 107 in the sequence listing and a light chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 239 of SEQ ID NO:
- 91 in the sequence listing.
A polypeptide selected from the group consisting of:
- 1) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 1 to 119 of SEQ ID NO:
- 35 in the sequence listing;
- 2) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 1 to 119 of SEQ ID NO:
- 39 in the sequence listing;
- 3) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 1 to 112 of SEQ ID NO:
- 37 in the sequence listing;
- 4) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 1 to 112 of SEQ ID NO:
- 41 in the sequence listing;
- 5) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 468 of SEQ ID NO:
- 107 in the sequence listing;
- 6) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 468 of SEQ ID NO:
- 115 in the sequence listing;
- 7) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 138 of SEQ ID NO:
- 107 in the sequence listing;
- 8) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 138 of SEQ ID NO:
- 115 in the sequence listing;
- 9) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 239 of SEQ ID NO:
- 91 in the sequence listing;
- 10) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 239 of SEQ ID NO:
- 99 in the sequence listing;
- 11) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 134 of SEQ ID NO:
- 91 in the sequence listing;
- 12) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 134 of SEQ ID NO:
- 99 in the sequence listing;
- 13) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 468 of SEQ ID NO:
- 139 in the sequence listing;
- 14) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 468 of SEQ ID NO:
- 147 in the sequence listing;
- 15) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 138 of SEQ ID NO:
- 139 in the sequence listing;
- 16) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 138 of SEQ ID NO:
- 147 in the sequence listing;
- 17) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 239 of SEQ ID NO:
- 123 in the sequence listing;
- 18) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 239 of SEQ ID NO:
- 131 in the sequence listing;
- 19) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 134 of SEQ ID NO:
- 123 in the sequence listing;
- and
- 20) a polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 134 of SEQ ID NO:
- 131 in the sequence listing.
The mouse hybridoma SH348-1 (FERM BP-10836).
An isolated antibody which specifically binds to EPHA2 consisting of a heavy chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 468 of SEQ ID NO:
- 115 in the sequence listing and a light chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 239 of SEQ ID NO:
- 99 in the sequence listing.
An isolated antibody which specifically binds to a polypeptide consisting of the amino acid sequence represented by SEQ ID NO:
- 8 in the sequence listing, wherein the antibody has the amino acid sequence represented by SEQ ID NO:
- 109 as CDRH1, SEQ ID NO:
- 111 as CDRH2, and SEQ ID NO:
- 113 as CDRH3, and has the amino acid sequence represented by SEQ ID NO:
- 93 as CDRL1, SEQ ID NO:
- 95 as CDRL2, and SEQ ID NO:
- 97 as CDRL3 in the sequence listing.
An isolated antibody which specifically binds to EPHA2 consisting of the following 1) and 2):
- 1) a heavy chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 138 of SEQ ID NO:
- 107 in the sequence listing or a heavy chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 138 of SEQ ID NO:
- 115 in the sequence listing;
- and
- 2) a light chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 134 of SEQ ID NO:
- 91 in the sequence listing or a light chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 134 of SEQ ID NO:
- 99 in the sequence listing.
An isolated antibody which specifically binds to EPHA2 consisting of a heavy chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 138 of SEQ ID NO:
- 107 in the sequence listing;
- and a light chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 134 of SEQ ID NO:
- 91 in the sequence listing.
An isolated antibody which specifically binds to EPHA2 consisting of a heavy chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 20 to 138 of SEQ ID NO:
- 115 in the sequence listing;
- and a light chain polypeptide comprising an amino acid sequence represented by amino acid Nos. 21 to 134 of SEQ ID NO:
- 99 in the sequence listing.
Dependencies are inferred from the claim text.