Composite membrane for western blot containing pvdf nanofiber and manufacturing method thereof
AssigneesAmomedi Co., Ltd., Amogreentech Co., Ltd.
InventorsChan KIM, Eu Gene CHO, Sang Chul SUH, In Yong SEO
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Abstract
Provided is a composite membrane for western blot, in which the composite membrane is prepared by combining nanofiber webs with nonwoven fabrics, and a basis weight of the nanofibers is in a range of 1 gsm to 50 gsm on the nonwoven fabrics, and an average pore size is in a range of 0.1 μm to 1.0 μm. The composite membrane for western blot including nanofibers has advantages such as saving of a production cost, and an excellent response characteristic due to a capillary phenomenon of a double structure, to thereby easily detect even a small amount of a particular substance present in a protein.
Claims12
A method of manufacturing a composite membrane for western blot, the composite membrane manufacturing method comprising the steps of:
- dissolving a polyvinilidenflouride (PVdF)-based polymer material in a solvent to prepare a spinning solution;
- obtaining webs of PVdF-based polymer nanofibers from the spinning solution by an electrospinning method;
- and
- combining the resulting nanofiber webs with nonwoven fabrics to obtain a composite membrane for western blot.
A composite membrane for western blot, the composite membrane that is prepared by combining nanofiber webs manufactured by an electrospinning method with nonwoven fabrics, wherein the thickness ratio of the nanofiber webs to the nonwoven fabrics combined with the nanofiber webs is in a range of approximately 1/15 to 1/10, the content of the nanofibers is in a range of 1 gsm to 50 gsm, and an average pore size is in a range of 0.1 μm to 1.0 μm.
Dependencies are inferred from the claim text.