Laser scanning microscope

A laser scanning microscope (LSM) for use with a sample, the LSM comprising:

• a microscope lens;
• at least one light source providing illuminating light, from which an illuminating beam path emanates in the direction of the sample;
• a detector arrangement of at least one detector;
• at least one detection beam path for transferring sample light to the detector arrangement;
• a first pinhole for first confocal filtering before the detector arrangement;
• a scanner for generating a relative movement between the illuminating light and the sample in at least one direction and through the microscope lens;
• wherein at least two illuminating beams are produced in the illumination beam path for illuminating the samples, the illumination beams being focused as illuminating points by the microscope lens in a sample plane, and wherein next to the first pinhole, which is designed as adjustable and slit-shaped with a variable slit diaphragm, a second, preferably adjustable slit-shaped pinhole, with a variable slit diaphragm, is arranged downstream from the first pinhole so as to be optically conjugate to the first pinhole and
• an image-rotating device for rotating the orientation of the illumination beams is arranged between the first and the second pinhole.