Extracorporeal clearance of organophosphates from blood on an acoustic separation device
AssigneeThe Charles Stark Draper Laboratory, Inc.
InventorsTammy Spain, Jason O. Fiering, Leila Albers
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Abstract
Systems, methods, and compositions for removing organophosphate toxins from blood are disclosed herein. The compositions include a lipid-based capture particle that displays BChE that binds to the toxin. The methods include acoustically separating toxins bound to lipid-based capture particles from blood factors of whole blood.
Claims22
A device for removing organophosphates from whole blood comprising:
- a microfluidic separation channel having an upstream end and downstream end, the separation channel comprising:
- a first inlet configured to introduce flowing whole blood into a proximal end of the separation channel, wherein the whole blood includes or is suspected to include organophosphates;
- a first outlet at the downstream end of the separation channel positioned substantially along the longitudinal axis of the separation channel;
- a second outlet at the downstream end positioned adjacent a first wall of the separation channel;
- and
- an acoustic transducer positioned adjacent to the separation channel for imposing a standing acoustic wave transverse to the flow of blood through a particle migration region of the separation channel;
- and
- a lipid-based capture particle injector containing lipid-based capture particles and configured to introduce lipid-based capture particles into the whole blood before the blood reaches the particle migration region of the separation channel, wherein the lipid-based capture particle comprises a first population of lipids and silicon oil;
- wherein the first population of lipids comprises organophosphate affinity molecules linked to the lipids in the first population of lipids.
A method of cleansing blood of a subject comprising:
- flowing whole blood into an inlet of a microfluidic separation channel wherein the whole blood comprises plasma and blood factors, and includes or is suspected to include organophosphates;
- introducing lipid-based capture particles into the whole blood which bind to the organophosphates, wherein the lipid-based capture particle comprises a first population of lipids and silicone oil;
- wherein the first population of lipids comprises an organophosphate affinity molecule linked to the lipids of the first population of lipids;
- applying a standing acoustic wave transverse to a direction of flow of the whole blood through the separation channel such that the blood factors aggregate to about the axial center of the separation channel and the lipid-based capture particles with bound organophosphates aggregate along at least one wall of the separation channel;
- and
A composition comprising organophosphate affinity molecules, a first population of lipids and silicon oil, wherein the organophosphates affinity molecules are linked to the first population of lipids, wherein the first population of lipids form a lipid-based capture particle, wherein the silicone oil is encapsulated within the lipid-based capture particle, and the organophosphate affinity molecules are displayed on the surface of the lipid-based capture particle.
Dependencies are inferred from the claim text.