# US 2014/0208458 A1 · Plant Having Complex Disease Resistance > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0208458 A1 - Document ID: US20140208458A1 - Status: Published application - Kind: A1 - Application number: 13/748,334 - Filed: 2013-01-23 - Published: 2014-07-24 - Earliest claimed: 2013-01-23 - Assignees: National Institute of Agrobiological Sciences - Applicants: National Institute of Agrobiological Sciences - Inventors: Hiroshi Takatsuji; Shingo Goto - Web page: https://patentgrep.com/patent/US20140208458A1 ## CPC - C12N 15/8261: {with agronomic (input) traits, e.g. crop yield} - C07K 14/415: from plants - C12N 15/8239: {pathogen inducible} - C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance} - C12N 15/8281: {for bacterial resistance} - C12N 15/8282: {for fungal resistance} - Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract The present invention relates to plants that have been transformed so as to have both disease resistance and acceptable agronomic traits. More specifically, the present invention relates to transgenic plants that have acquired disease resistance through expression in the plants of a polynucleotide encoding the transcription factor WRKY45 in an infection-responsive manner, and methods for generating the transgenic plants. ## Claims ### Claim 1 A transgenic plant comprising a functional WRKY45 gene, wherein the WRKY45 gene is operably linked to an infection-responsive promoter and a translational enhancer. ### Claim 2 (depends on 1) The transgenic plant of claim 1, wherein the WRKY45 gene is the WRKY45 gene of rice. ### Claim 3 (depends on 2) The transgenic plant of claim 2, wherein the WRKY45 gene has a nucleotide sequence encoding an amino acid sequence as set forth in SEQ ID NO: 2, or an amino acid sequence at least 95% identical to the sequence of SEQ ID NO: 2. ### Claim 4 (depends on 1) The transgenic plant of claim 1, wherein the infection-responsive promoter directs transcription of the WRKY45 gene within six hours of infection. ### Claim 5 (depends on 1) The transgenic plant of claim 1, wherein the infection-responsive promoter is the PR1b gene promoter. ### Claim 6 (depends on 5) The transgenic plant of claim 5, wherein the PR1b gene promoter has a nucleotide sequence as set forth in SEQ ID NO: 3, or a nucleotide sequence at least 95% identical to the sequence of SEQ ID NO: 3. ### Claim 7 (depends on 1) The transgenic plant of claim 1, wherein the infection-responsive promoter is the GST gene promoter. ### Claim 8 (depends on 7) The transgenic plant of claim 7, wherein the GST gene promoter has a nucleotide sequence as set forth in SEQ ID NO: 4, or a nucleotide sequence at least 95% identical to the sequence of SEQ ID NO: 4. ### Claim 9 (depends on 1) The transgenic plant of claim 1, wherein the translational enhancer is an alcohol dehydrogenase 5′-untranslated region (ADH-UTR). ### Claim 10 (depends on 9) The transgenic plant of claim 9, wherein the alcohol dehydrogenase 5′-untranslated region (ADH-UTR) has a nucleotide sequence as set forth in SEQ ID NO: 5, or a nucleotide sequence at least 95% identical to the sequence of SEQ ID NO: 5. ### Claim 11 (depends on 1) The transgenic plant of claim 1, wherein the WRKY45 gene is further operably linked to a tandem terminator comprising two terminator sequences in tandem. ### Claim 12 (depends on 11) The transgenic plant of claim 11, wherein the tandem terminator comprises the nos terminator and Cauliflower mosaic virus 35S terminator. ### Claim 13 (depends on 12) The transgenic plant of claim 12, wherein the tandem terminator has a nucleotide sequence as set forth in SEQ ID NO: 6, or a nucleotide sequence at least 95% identical to the sequence of SEQ ID NO: 6. ### Claim 14 (depends on 1) The transgenic plant of claim 1, wherein the infection-responsive promoter is responsive to infection by a filamentous fungus. ### Claim 15 (depends on 1) The transgenic plant of claim 1, wherein the infection-responsive promoter is responsive to infection by a bacterium. ### Claim 16 (depends on 1) The transgenic plant of claim 1, wherein the plant shows complex disease resistance. ### Claim 17 (depends on 1) The transgenic plant of claim 1, wherein the plant has agronomic traits that are at least comparable to those of a naturally-occurring plant. ### Claim 18 (depends on 1) The transgenic plant of claim 1, wherein the transgenic plant is a monocot. ### Claim 19 (depends on 1) The transgenic plant of claim 1, wherein the transgenic plant is rice. ### Claim 20 (depends on 1) A transgenic plant which is a progeny or clone of the transgenic plant of claim 1. ### Claim 21 (depends on 1) A propagation material of the transgenic plant of claim 1. ### Claim 22 An isolated nucleic acid construct comprising a functional WRKY45 gene, wherein the WRKY45 gene is operably linked to an infection-responsive promoter and a translational enhancer. ### Claim 23 (depends on 22) A vector comprising the isolated nucleic acid construct of claim 22. ### Claim 24 (depends on 23) A plant cell into which the vector of claim 23 has been introduced. ### Claim 25 (depends on 22) A method for producing a transgenic plant, comprising the steps of: a) introducing the isolated nucleic acid construct of claim 22 into a plant cell; and b) regenerating a plant from the plant cell. ### Claim 26 (depends on 22) A method for improving plant disease resistance, comprising introducing the isolated nucleic acid construct of claim 22 into a plant. ### Claim 27 (depends on 22) An agent for improving plant disease resistance, wherein the agent comprises the isolated nucleic acid construct of claim 22. ### Claim 28 A method for producing a gene product in a plant in an infection-inducible manner, comprising: providing a plant comprising a gene of interest operably linked to a GST gene promoter, wherein the GST gene promoter directs transcription of a gene of interest upon infection to produce the gene product.