# US 2014/0208453 A1 · Soybean plants having superior agronomic performance and methods for their production

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2014/0208453 A1
- Document ID: US20140208453A1
- Status: Published application
- Kind: A1
- Application number: 14/134,854
- Filed: 2013-12-19
- Published: 2014-07-24
- Earliest claimed: 2003-08-01
- Assignees: Pioneer Hi-Bred International, Inc.
- Applicants: Pioneer Hi-Bred International, Inc.
- Inventors: SCOTT SEBASTIAN
- Web page: https://patentgrep.com/patent/US20140208453A1

## CPC

- A01H 1/045: {using molecular markers}
- A01H 5/10: Seeds
- A01H 6/542: {Glycine max [soybean]}
- C12Q 1/6895: for plants, fungi or algae
- C12Q 2600/13: Plant traits
- C12Q 2600/156: Polymorphic or mutational markers
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

This invention provides compositions including favorable alleles of marker loci associated with genetic elements contributing to superior agronomic performance. Also provided are markers for identifying favorable alleles of marker loci associated with genetic elements involved in superior agronomic performance, as well as methods employing the markers.

## Claims

### Claim 1

A method of identifying a soybean subline with increased yield, the method comprising: determining in a genome of each of a plurality of progeny of a progenitor soybean, at least one allelic form of between about 10% and about 100% of the chromosome segments each comprising a genetic element contributing to increased yield and each comprising or proximal to a marker locus selected from the set of marker loci consisting of: Satt684, Satt165, Satt042, Satt364, Satt454, Satt526, Satt300, Satt591, Satt155, Satt385, Satt225, Satt236, Satt511, P12390B-1, Satt480, Satt632-TB, Satt233, Satt327, Satt329, Satt508, P10635A-1, Satt409, Satt228, Satt429, Satt426, Satt509, SAT—261, Satt197, Satt519, Satt597, SCT—026, Satt415, Satt583, Satt430, P12198A-1, P8584A-1, Satt359, P10648A-1, P12105A-1, P10641A-1, Satt168, Satt556, Satt272, Satt020, Satt066, Satt534, P10638B-2, Satt399, Satt361, P10639A-1, Satt661-TB, Satt190, SAT—311-DB, Satt338, Satt227, Satt640-TB, Satt422, Satt457, Satt557, Satt319, SAT—142-DB, Satt460, P13073A-1, Satt307, SCT-028, Satt433, Satt357, Satt321, Satt267, Satt383, Satt295, Satt203, Satt507, SAT—110, P10620A-1, Satt129, Satt147, Satt216, SAT—351, P10621B-2, Satt701, Satt634, Satt558, Satt266, Satt282, Satt537, Satt506, Satt546, P13072A-1, Satt582, Satt389, Satt461, Satt311, Satt514, Satt464, Satt662, Satt543, Satt186, Satt413, Satt672, P13074A-1, P10624A-1, Satt573, Satt598, Satt204, Satt263, Satt491, Satt602, Satt151, Satt355, Satt452, SAT—273-DB, Satt146, Satt193, Satt569, Satt343, Satt586, Satt040, Satt423, Satt348, Satt595, P10782A-1, P3436A-1, P10598A-1, Satt334, Satt510, Satt144, Satt522, P9026A-1, P10646A-1, P5219A-1, P7659A-2, Satt570, Satt356, Satt130, Satt115, Satt594, Satt533, Satt303, Satt352, Satt566, Satt199, Satt503, Satt517, Satt191, SAT—117, Satt353, Satt442, Satt279, Satt314, Satt142, Satt181, Satt367, Satt127, SCTT012, Satt270, Satt292, Satt440, P10640A-1, Satt249, SCT—065, Satt596, Satt280, Satt406, Satt380, Satt183, Satt529, Satt431, Satt242, Satt102, Satt441, Satt544, Satt617, Satt240, P10618A-1, Satt475, Satt196, SAT—301, Satt523, Satt418, Satt398, Satt497, Satt284, Satt166, Satt448, Satt373, Satt513, P12394A-1, Satt590, Satt567, Satt220, Satt536, Satt175, Satt677, Satt680, P10615A-1, Satt551, Satt250, Satt346, Satt336, SAT—330-DB, P13069A-1, P5467A-1, P5467A-2, Satt584, SAT—084, P3050A-2, SAT—275-DB, Satt387, Satt549, Satt660, Satt339, Satt255, Satt257, Satt358, P12396A-1, Satt487, Satt259, Satt347, Satt420, Satt576, Satt550, Satt633, Satt262, Satt473, Satt477, Satt581, P11070A-1, Satt153, Satt243, P8230A-1, P10623A-1, P10632A-1, P10793A-1, P12391A-1, P13560A-1, P13561A-1, P2481A-1, Satt040, Satt108, Satt109, Satt111, Satt176, Satt219, Satt299, and Satt512; wherein the favorable allelic form of at least one chromosome segment is determined by: a) identifying at least one polymorphic marker locus selected from the set in a plurality of sublines of progeny of a progenitor soybean; b) assessing yield in at least two sublines of progeny, which sublines of progeny have different allelic forms of at least one marker locus; and, c) identifying the subline of progeny with increased yield relative to the progenitor soybean; thereby identifying a subline with increased yield.

### Claim 2 (depends on 1)

The method of claim 1, wherein the at least two sublines of progeny are near isogenic sublines.

### Claim 3 (depends on 1)

The method of claim 1, wherein the at least two sublines of progeny are random sublines.

### Claim 4 (depends on 1)

The method of claim 1, wherein the plurality of progeny are obtained by selfing a progenitor soybean.

### Claim 5 (depends on 1)

The method of claim 1, wherein the plurality of progeny are obtained by crossing a first progenitor soybean and a second progenitor soybean.

### Claim 6 (depends on 5)

The method of claim 5, wherein a first progenitor soybean comprising a strain of elite germplasm is crossed with a second progenitor soybean comprising a different strain of elite germplasm to generate a population of soybean plants from which the progeny is selected.

### Claim 7 (depends on 5)

The method of claim 5, wherein a first progenitor soybean comprising a strain of elite germplasm is crossed with a second progenitor soybean comprising a strain of exotic germplasm to generate a population of soybean plants from which the progeny is selected.

### Claim 8 (depends on 6)

The method of claim 6, wherein the elite strain of germplasm is selected from the group consisting of: 90A07, 90B11, 90B31, 90B43, 90B72, 90B73, 91B01, 94B53, 94B54, 94B73, 95B32, 95B33, 95B34, 95B53, 95B95, 95B96, 95B97, 96B21, 96B51, 97B52, 97B61, BEDFORD, CX105, CX232, CX253, CX289, CX394C, CX469C, ESSEX, EX04C00, FORREST, HS93-4118, HUTCHESON, JIM, KORADA, PHARAOH, S0066, S0880, S1990, S19T9, S20F8, S25J5, S32Z3, S33N1, S38T8, S3911, S4260, S42H1, S43B5, S5960, S6262, ST0653, ST1073, ST1090, ST1570, ST1690, ST1970, ST2250, ST2488, ST2660, ST2686, ST2688, ST2788, ST2870, ST3171, ST3630, ST3660, ST3870, ST3883, TRACY, TRAILL, and YOUNG.

### Claim 9 (depends on 1)

The method of claim 1, further comprising selecting at least one plant of the identified soybean subline.

### Claim 10 (depends on 9)

The method of claim 9, wherein the selected soybean plant comprises a whole plant, a plant organ, a plant seed, a plant cell or a plant tissue culture.

### Claim 11 (depends on 9)

The method of claim 9, wherein the selected soybean plant or a progeny thereof is crossed with a second soybean plant, which second soybean plant lacks the determined alleles of marker loci or the determined allelic forms of the plurality of chromosome segments.

### Claim 12

A plant comprising in its genome a set of genetic elements related to increased yield, wherein said set of genetic elements comprises one or more genetic elements proximal to each of at least 10% of the markers selected from the group consisting of:
Satt684, Satt165, Satt042, Satt364, Satt454, Satt526, Satt300, Satt591, Satt155, Satt385, Satt225, Satt236, Satt511, P12390B-1, Satt480, Satt632-TB, Satt233, Satt327, Satt329, Satt508, P10635A-1, Satt409, Satt228, Satt429, Satt426, Satt509, SAT—261, Satt197, Satt519, Satt597, SCT—026, Satt415, Satt583, Satt430, P12198A-1, P8584A-1, Satt359, P10648A-1, P12105A-1, P10641A-1, Satt168, Satt556, Satt272, Satt020, Satt066, Satt534, P10638B-2, Satt399, Satt361, P10639A-1, Satt661-TB, Satt190, SAT—311-DB, Satt338, Satt227, Satt640-TB, Satt422, Satt457, Satt557, Satt319, SAT—142-DB, Satt460, P13073A-1, Satt307, SCT—028, Satt433, Satt357, Satt321, Satt267, Satt383, Satt295, Satt203, Satt507, SAT—110, P10620A-1, Satt129, Satt147, Satt216, SAT—351, P10621B-2, Satt701, Satt634, Satt558, Satt266, Satt282, Satt537, Satt506, Satt546, P13072A-1, Satt582, Satt389, Satt461, Satt311, Satt514, Satt464, Satt662, Satt543, Satt186, Satt413, Satt672, P13074A-1, P10624A-1, Satt573, Satt598, Satt204, Satt263, Satt491, Satt602, Satt151, Satt355, Satt452, SAT—273-DB, Satt146, Satt193, Satt569, Satt343, Satt586, Satt040, Satt423, Satt348, Satt595, P10782A-1, P3436A-1, P10598A-1, Satt334, Satt510, Satt144, Satt522, P9026A-1, P10646A-1, P5219A-1, P7659A-2, Satt570, Satt356, Satt130, Satt115, Satt594, Satt533, Satt303, Satt352, Satt566, Satt199, Satt503, Satt517, Satt191, SAT—117, Satt353, Satt442, Satt279, Satt314, Satt142, Satt181, Satt367, Satt127, SCTT012, Satt270, Satt292, Satt440, P10640A-1, Satt249, SCT—065, Satt596, Satt280, Satt406, Satt380, Satt183, Satt529, Satt431, Satt242, Satt102, Satt441, Satt544, Satt617, Satt240, P10618A-1, Satt475, Satt196, SAT—301, Satt523, Satt418, Satt398, Satt497, Satt284, Satt166, Satt448, Satt373, Satt513, P12394A-1, Satt590, Satt567, Satt220, Satt536, Satt175, Satt677, Satt680, P10615A-1, Satt551, Satt250, Satt346, Satt336, SAT—330-DB, P13069A-1, P5467A-1, P5467A-2, Satt584, SAT—084, P3050A-2, SAT—275-DB, Satt387, Satt549, Satt660, Satt339, Satt255, Satt257, Satt358, P12396A-1, Satt487, Satt259, Satt347, Satt420, Satt576, Satt550, Satt633, Satt262, Satt473, Satt477, Satt581, P11070A-1, Satt153, Satt243, P8230A-1, P10623A-1, P10632A-1, P10793A-1, P12391A-1, P13560A-1, P13561A-1, P2481A-1, Satt040, Satt108, Satt109, Satt111, Satt176, Satt219, Satt299, and Satt512.

### Claim 13 (depends on 12)

The plant of claim 12, wherein said set of genetic elements comprises one or more genetic elements proximal to each of at least 50% of the markers selected from the group consisting of:
Satt684, Satt165, Satt042, Satt364, Satt454, Satt526, Satt300, Satt591, Satt155, Satt385, Satt225, Satt236, Satt511, P12390B-1, Satt480, Satt632-TB, Satt233, Satt327, Satt329, Satt508, P10635A-1, Satt409, Satt228, Satt429, Satt426, Satt509, SAT—261, Satt197, Satt519, Satt597, SCT—026, Satt415, Satt583, Satt430, P12198A-1, P8584A-1, Satt359, P10648A-1, P12105A-1, P10641A-1, Satt168, Satt556, Satt272, Satt020, Satt066, Satt534, P10638B-2, Satt399, Satt361, P10639A-1, Satt661-TB, Satt190, SAT—311-DB, Satt338, Satt227, Satt640-TB, Satt422, Satt457, Satt557, Satt319, SAT—142-DB, Satt460, P13073A-1, Satt307, SCT—028, Satt433, Satt357, Satt321, Satt267, Satt383, Satt295, Satt203, Satt507, SAT—110, P10620A-1, Satt129, Satt147, Satt216, SAT—351, P10621B-2, Satt701, Satt634, Satt558, Satt266, Satt282, Satt537, Satt506, Satt546, P13072A-1, Satt582, Satt389, Satt461, Satt311, Satt514, Satt464, Satt662, Satt543, Satt186, Satt413, Satt672, P13074A-1, P10624A-1, Satt573, Satt598, Satt204, Satt263, Satt491, Satt602, Satt151, Satt355, Satt452, SAT—273-DB, Satt146, Satt193, Satt569, Satt343, Satt586, Satt040, Satt423, Satt348, Satt595, P10782A-1, P3436A-1, P10598A-1, Satt334, Satt510, Satt144, Satt522, P9026A-1, P10646A-1, P5219A-1, P7659A-2, Satt570, Satt356, Satt130, Satt115, Satt594, Satt533, Satt303, Satt352, Satt566, Satt199, Satt503, Satt517, Satt191, SAT—117, Satt353, Satt442, Satt279, Satt314, Satt142, Satt181, Satt367, Satt127, SCTT012, Satt270, Satt292, Satt440, P10640A-1, Satt249, SCT—065, Satt596, Satt280, Satt406, Satt380, Satt183, Satt529, Satt431, Satt242, Satt102, Satt441, Satt544, Satt617, Satt240, P10618A-1, Satt475, Satt196, SAT—301, Satt523, Satt418, Satt398, Satt497, Satt284, Satt166, Satt448, Satt373, Satt513, P12394A-1, Satt590, Satt567, Satt220, Satt536, Satt175, Satt677, Satt680, P10615A-1, Satt551, Satt250, Satt346, Satt336, SAT—330-DB, P13069A-1, P5467A-1, P5467A-2, Satt584, SAT—084, P3050A-2, SAT—275-DB, Satt387, Satt549, Satt660, Satt339, Satt255, Satt257, Satt358, P12396A-1, Satt487, Satt259, Satt347, Satt420, Satt576, Satt550, Satt633, Satt262, Satt473, Satt477, Satt581, P11070A-1, Satt153, Satt243, P8230A-1, P10623A-1, P10632A-1, P10793A-1, P12391A-1, P13560A-1, P13561A-1, P2481A-1, Satt040, Satt108, Satt109, Satt111, Satt176, Satt219, Satt299, and Satt512.

### Claim 14 (depends on 12)

The plant of claim 12, wherein said set of genetic elements comprises one or more genetic elements proximal to each of at least 10% of the markers selected from the group consisting of:
Satt642, Satt042, Satt364, Satt454, Satt526, Satt300, Satt591, Satt155, Satt385, Satt511, P12390B-1, Satt632-TB, Satt429, SAT—261, Satt197, P10641A-1, Satt556, Satt534, P10638B-2, Satt399, Satt361, P10639A-1, Satt661-TB, Satt190, SAT—311-DB, Satt338, Satt640-TB, Satt557, Satt319, SAT—142-DB, Satt460, Satt433, Satt357, Satt321, Satt295, Satt203, Satt507, Satt129, Satt147, SAT—351, P10621B-2, Satt558, Satt701, Satt634, Satt582, Satt389, Satt464, Satt662, Satt672, Satt573, Satt598, Satt263, Satt602, Satt151, SAT—273-DB, Satt146, Satt193, Satt569, Satt176, Satt343, Satt586, Satt040, Satt595, P10782A-1, Satt334, Satt144, Satt522, Satt570, Satt356, Satt533, Satt199, Satt517, Satt191, SAT—117, Satt279, Satt181, Satt127, Satt270, Satt292, SAT—065, Satt596, Satt406, Satt380, Satt183, Satt529, Satt242, Satt617, Satt240, SAT—301, Satt418, Satt398, Satt497, Satt166, Satt448, Satt373, Satt513, P12394A-1, Satt536, Satt175, Satt677, Satt680, P10615A-1, Satt551, Satt346, Satt336, SAT—330-DB, P13069A-1, P5467A-1, P5467A-2, SAT—084, SAT—275-DB, Satt660, Satt339, P12396A-1, Satt358, Satt487, Satt259, Satt420, Satt576, Satt633, Satt477, Satt581, Satt153, Satt243, P10793A-1, P12391A-1, P12392A-1, P13560A-1, P13561A-1, Satt040, Satt111, Satt176, Satt219, and Satt299.

### Claim 15 (depends on 12)

The plant of claim 12, wherein said set of genetic elements comprises one or more genetic elements proximal to each of at least 10% of the markers selected from the group consisting of:
Satt684, Satt042, Satt364, Satt454, Satt526, Satt300, Satt591, Satt155, Satt385, Satt632-TB, Satt429, SAT—261, P10641A-1, Satt556, P10638B-2, Satt399, Satt361, Satt661-TB, Satt190, SAT—311-DB, Satt338, Satt640-TB, Satt557, Satt319, SAT—142-DB, Satt321, Satt203, Satt129, Satt147, SAT—351, P10621B-2, Satt701, Satt634, Satt582, Satt389, Satt464, Satt662, Satt672, Satt573, Satt598, Satt263, Satt151, SAT—273-DB, Satt146, Satt193, Satt569, Satt343, Satt586, Satt040, Satt595, Satt334, Satt144, Satt522, Satt570, Satt356, Satt199, Satt517, Satt191, SAT—117, Satt279, Satt181, Satt127, Satt270, Satt292, Sat—065, Satt596, Satt406, Satt380, Satt183, Satt529, Satt242, Satt617, Satt240, SAT—301, Satt418, Satt398, Satt497, Satt166, Satt448, Satt373, Satt513, P12394A-1, Satt536, Satt175, Satt677, Satt680, P10615A-1, Satt551, SAT—330-DB, P13069A-1, P5467A-1, P5467A-2, SAT—084, SAT—275-DB, Satt660, Satt339, Satt358, Satt487, Satt420, Satt576, Satt633, Satt581, Satt153, Satt243, P10793A-1, P13560A-1, P13561A-1, Satt111, Satt219, and Satt299.

### Claim 16 (depends on 12)

The plant of claim 12, wherein said set of genetic elements comprises one or more genetic elements proximal to each of at least 50% of the markers selected from the group consisting of:
Satt684, Satt526, Satt591, Satt385, Satt632-TB, Satt429, SAT—261, P10641A-1, Satt556, P10638B-2, Satt190, SAT—311-DB, Satt338, Satt640-TB, Satt557, SAT—142-DB, Satt321, Satt203, Satt129, SAT—351, Satt701, Satt582, Satt389, Satt464, Satt672, Satt598, Satt343, Satt595, Satt334, Satt144, Satt522, Satt570, Satt356, Satt199, Sat—117, Satt279, Satt181, Satt127, Satt270, Satt292, Sat—065, Satt529, Satt242, Satt617, SAT—301, Satt398, Satt497, Satt166, Satt373, Satt680, P10615A-1, SAT—330-DB, P13069A-1, SAT—275-DB, Satt339, Satt487, Satt420, Satt58, 1 and Satt153.

### Claim 17 (depends on 12)

The plant of claim 12, wherein said set of genetic elements comprises one or more genetic elements proximal to each of the markers:
Satt526, Satt591, Satt429, SAT-—261, P10641A-1, Satt190, Satt557, SAT—142-DB, Satt129, SAT—351, Satt464, Satt343, Satt595, Satt570, Satt181, Satt127, Satt270, Sat—065, Satt529, Satt242, Satt398, Satt166, Satt373, Satt680, SAT—330-DB, P13069A-1, Satt339, Satt487, Satt581, and Satt153.

### Claim 18 (depends on 12)

The plant of claim 12, wherein the plant is a soybean line or soybean variety.

### Claim 19 (depends on 17)

The plant of claim 17, wherein the plant is a soybean line or soybean variety.

### Claim 20 (depends on 12)

The plant of claim 12, wherein the plant comprises an elite soybean strain or an exotic soybean strain.

### Claim 21 (depends on 17)

The plant of claim 17, wherein the plant comprises an elite soybean strain or an exotic soybean strain.

### Claim 22 (depends on 12)

A field comprising a plurality of plants of claim 12.

### Claim 23 (depends on 17)

A field comprising a plurality of plants of claim 17.

### Claim 24 (depends on 12)

A seed of the plant of claim 12.

### Claim 25 (depends on 17)

A seed of the plant of claim 17.

### Claim 26 (depends on 24)

A method of producing a plant having increased yield, the method comprising planting the seed of claim 24 in soil and growing a plant therefrom.

### Claim 27 (depends on 25)

A method of producing a plant having increased yield, the method comprising planting the seed of claim 25 in soil and growing a plant therefrom.