# US 2014/0197309 A1 · Systems and methods of detecting and demonstrating hair damage via evaluation of protein fragments

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- Number: US 2014/0197309 A1
- Document ID: US20140197309A1
- Status: Published application
- Kind: A1
- Application number: 14/217,977
- Filed: 2014-03-18
- Published: 2014-07-17
- Earliest claimed: 2010-05-17
- Assignees: The Procter & Gamble Company
- Applicants: The Procter & Gamble Company
- Inventors: Michael Glenn Davis; Michael Joseph Flagler; Yiping Sun; Tanuja Chaudhary
- Web page: https://patentgrep.com/patent/US20140197309A1

## CPC

- G01N 33/4833: {of solid biological material, e.g. tissue samples, cell cultures (tissue in vivo A61B5/00; cell suspensions G01N33/48735)}
- G01N 33/6839: {involving dyes, e.g. Coomassie blue, bromcresol green}
- G01N 33/6851: {Methods of protein analysis involving laser desorption ionisation mass spectrometry}
- H01J 49/0036: {Step by step routines describing the handling of the data generated during a measurement}
- G01N 33/6824: {involving N-terminal degradation, e.g. Edman degradation}
- G01N 33/6848: {Methods of protein analysis involving mass spectrometry}
- G01N 2333/4742: Keratin; Cytokeratin
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H)

## Abstract

Embodiments of a method for demonstrating type and/or source of hair damage comprises extracting protein fragments from a hair sample with an aqueous solution, testing the resulting protein fragments with the MALDI-MS test, and then either comparing the results between a damaged sample and an undamaged sample or comparing the results between a damaged sample and a list of known marker protein fragments to identify the type and/or source of the damage.

## Claims

### Claim 1

A method for demonstrating hair damage type or source, the method comprising:
a) extracting the labile proteins using a suitable solvent from a hair sample;
b) analyzing the protein fragments sample with MALDI-MS; resulting in protein fragment results;
c) identifying the hair damage by comparing the protein fragments results to a list of marker protein fragments for particular damage.

### Claim 2 (depends on 1)

The method of claim 1, wherein the MALDI-MS protein fragment results include a +28 and +71 modification to the N-terminus of the protein fragment having the structure: