# US 2014/0197107 A1 · Method of purifying nucleic acids, method of extracting nucleic acids and kit for purifying nucleic acids > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0197107 A1 - Document ID: US20140197107A1 - Status: Published application - Kind: A1 - Application number: 14/239,340 - Filed: 2012-08-28 - Published: 2014-07-17 - Earliest claimed: 2011-09-13 - Assignees: Sony Corporation - Inventors: Tomohiko Nakamura; Naohisa Sakamoto; Tasuku Yotoriyama; Kazumine Ito - Web page: https://patentgrep.com/patent/US20140197107A1 ## CPC - C12N 15/101: {by chromatography, e.g. electrophoresis, ion-exchange, reverse phase} - B01D 15/34: Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation - B01D 15/362: {Cation-exchange} - B01D 15/363: {Anion-exchange} - B01J 39/04: Processes using organic exchangers - B01J 41/04: Processes using organic exchangers - C12N 15/09: Recombinant DNA-technology - C12Q 1/68: involving nucleic acids - C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) - G01N 30/88: Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 - G01N 30/96: using ion-exchange (G01N30/02, G01N30/90 take precedence) - G01N 2030/8827: {involving nucleic acids} - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28) - B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract [Object] To provide a method of purifying nucleic acids where the operation is simple and the nucleic acids can be extracted in a short time with high efficiency. [Solving Means] A method of purifying nucleic acids including the step of adsorbing substances in a sample containing nucleic acids with an ion exchange resin 10 including a positive ion exchange resin and a negative ion exchange resin. As the positive ion exchange resin, a first positive ion exchange resin and a second positive ion exchange resin having an exclusion limit molecular weight lower than that of the first positive ion exchange resin may be used. ## Claims ### Claim 1 A method of purifying nucleic acids, comprising the step of: adsorbing substances in a sample containing nucleic acids with an ion exchange resin including a positive ion exchange resin and a negative ion exchange resin. ### Claim 2 (depends on 1) The method of purifying nucleic acids according to claim 1, wherein the positive ion exchange resin includes a first positive ion exchange resin and a second positive ion exchange resin having an exclusion limit molecular weight lower than that of the first positive ion exchange resin. ### Claim 3 (depends on 2) The method of purifying nucleic acids according to claim 2, wherein the substances are adsorbed by the first positive ion exchange resin and then are further adsorbed by the negative ion exchange resin and the second positive ion exchange resin. ### Claim 4 (depends on 3) The method of purifying nucleic acids according to claim 3, wherein the sample is flowed into a column including the first positive ion exchange resin in an upper layer and the negative ion exchange resin and the second positive ion exchange resin in a lower layer from an upper layer side. ### Claim 5 (depends on 4) The method of purifying nucleic acids according to claim 4, wherein the step is for adsorbing the substances included in the sample that is diluted with a buffer solution by the ion exchange resin, and the buffer solution has a pH of 4.0 to 8.0. ### Claim 6 (depends on 5) The method of purifying nucleic acids according to claim 5, wherein the positive ion exchange resin is a strong acidic positive ion exchange resin. ### Claim 7 (depends on 6) The method of purifying nucleic acids according to claim 6, wherein a counter ion of the first positive ion exchange resin is Na+. ### Claim 8 (depends on 7) The method of purifying nucleic acids according to claim 7, wherein a counter ion of the first positive ion exchange resin is H+. ### Claim 9 (depends on 8) The method of purifying nucleic acids according to claim 8, wherein the negative ion exchange resin is a strong basic negative ion exchange resin. ### Claim 10 (depends on 9) The method of purifying nucleic acids according to claim 9, wherein a counter ions of the negative ion exchange resin is OH−. ### Claim 11 (depends on 10) The method of purifying nucleic acids according to claim 10, wherein a percentage of an ion exchange capacity of the negative ion exchange resin to the second positive ion exchange resin is 50% to 150%. ### Claim 12 (depends on 11) The method of purifying nucleic acids according to claim 11, wherein a nonionic surfactant and/or a nonionic hydrophilic polymer compound is used to adsorb the substances. ### Claim 13 (depends on 12) The method of purifying nucleic acids according to claim 12, wherein the substances are foreign substances containing at least a protein and a metal salt. ### Claim 14 A method of extracting nucleic acids, comprising the steps of: ultrasonically treating the sample including nucleic acids, adsorbing substances included in the sample with a positive ion exchange resin and a negative ion exchange resin, and concentrating the nucleic acids by blocking the nucleic acids migrated by electrophoresis. ### Claim 15 (depends on 14) The method of extracting nucleic acids according to claim 14, wherein the electrophoresis is carried out on the nucleic acids into which an intercalator having an anionic functional group is inserted. ### Claim 16 (depends on 15) The method of extracting nucleic acids according to claim 15, wherein the electrophoresis is carried out on the nucleic acids by mixing the sample, a compound having a functional group that is reacted with a carboxyl group of the substances included in the sample by dehydration condensation, and a condensation agent of the dehydration condensation reaction. ### Claim 17 A kit for purifying nucleic acids comprising: a positive ion exchange resin; a negative ion resin; and a nucleic acid purifying instrument internally holding the positive ion exchange resin and the negative ion exchange resin for distributing a sample including nucleic acids.