# US 2014/0197100 A1 · Methods and compositions for reducing bisphenol a release

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- Number: US 2014/0197100 A1
- Document ID: US20140197100A1
- Status: Published application
- Kind: A1
- Application number: 13/978,239
- Filed: 2012-07-11
- Published: 2014-07-17
- Earliest claimed: 2012-07-11
- Assignees: Empire Technology Development LLC
- Inventors: Yanjie Xu; Robert Matthew Cabrera
- Web page: https://patentgrep.com/patent/US20140197100A1

## CPC

- C02F 1/70: by reduction {(C02F1/4676 takes precedence)}
- C02F 3/108: {Immobilising gels, polymers or the like}
- C02F 3/342: {characterised by the enzymes used}
- C08G 77/06: Preparatory processes
- C08G 77/26: nitrogen-containing groups
- C08L 83/06: containing silicon bound to oxygen-containing groups (C08L83/12 takes precedence)
- C08L 83/08: containing silicon bound to organic groups containing atoms other than carbon, hydrogen and oxygen
- C12N 11/089: obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- C02F 2101/345: {Phenols}
- C08K 2201/019: the composition being defined by the absence of a certain additive
- Y02W 10/10: Biological treatment of water, waste water, or sewage
- C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes)
- C08G: MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08L: COMPOSITIONS OF MACROMOLECULAR COMPOUNDS (compositions based on polymerisable monomers C08F, C08G; artificial filaments or fibres D01F; textile treating compositions D06)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C08K: Use of inorganic or non-macromolecular organic substances as compounding ingredients (paints, inks, varnishes, dyes, polishes, adhesives C09)
- Y02W: CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT

## Abstract

Disclosed are methods, compositions and systems pertaining to polymer coatings that entrap enzymes, specifically enzymes capable of reducing carboxylic acids.

## Claims

### Claim 1

A carboxylic acid-reducing composition comprising:
at least one carboxylic acid-reducing enzyme and a polymer, wherein the polymer comprises organic silane.

### Claim 2 (depends on 1)

The composition of claim 1, wherein the carboxylic acid-reducing enzyme is entrapped in the polymer.

### Claim 3 (depends on 1)

The composition of claim 1, wherein the organic silane is formed from polymerization of methyltrimethoxysilane and gamma-aminopropyltriethoxysilane.

### Claim 4 (depends on 1)

The composition of claim 1, wherein at least one carboxylic acid-reducing enzyme comprises one or more of carboxylate reductase, aldehyde ferredoxin oxidoreductase, formaldehyde ferredoxin oxidoreductase, glyceraldehyde-3-phosphate ferredoxin oxidoreductase, and (2R)-hydroxycarboxylate-viologen-oxidoreductase.

### Claim 5 (depends on 1)

The composition of claim 1, wherein the composition is in the form of a non-perforated sheet, a perforated sheet, beads, dry particulate, or gel.

### Claim 6 (depends on 1)

The composition of claim 1, wherein the composition is in the form of a gel suspended in an adhesive film.

### Claim 7

A method of treating a composition comprising a bisphenol A (BPA) precursor, the method comprising:
contacting the composition comprising the BPA precursor with a carboxylic acid-reducing composition, wherein the carboxylic acid-reducing composition comprises a carboxylic acid-reducing enzyme and organic silane, and
wherein the BPA precursor comprises a polymer that produces BPA monomers upon polymer hydrolysis.

### Claim 8 (depends on 7)

The method of claim 7, wherein the BPA precursor comprises polycarbonate, polyester carbonate, or an epoxy resin.

### Claim 9 (depends on 7)

The method of claim 7, wherein the organic silane is formed from polymerization of methyltrimethoxysilane and gamma-aminopropyltriethoxysilane.

### Claim 10 (depends on 7)

The composition of claim 7, wherein the carboxylic acid-reducing enzyme is entrapped in the organic silane.

### Claim 11 (depends on 7)

The method of claim 7, wherein at least one carboxylic acid-reducing enzyme is selected from carboxylate reductase, aldehyde ferredoxin oxidoreductase, formaldehyde ferredoxin oxidoreductase, glyceraldehyde-3-phosphate ferredoxin oxidoreductase, and (2R)-hydroxycarboxylate-viologen-oxidoreductase.

### Claim 12 (depends on 7)

The method of claim 7, wherein the carboxylic acid-reducing composition is in the form of a gel suspended in an adhesive film.

### Claim 13 (depends on 7)

The method of claim 7, and wherein the carboxylic acid-reducing composition is contacted with at least a portion of the composition comprising the BPA precursor.

### Claim 14 (depends on 7)

The method of claim 7, wherein contacting comprises polymerizing the carboxylic acid-reducing composition directly onto at least a portion of the composition comprising the BPA precursor.

### Claim 15

A method of treating water that contains 2,2-bis(4-hydroxyphenyl)propanoic acid (BPA-COOH), the method comprising:
contacting the water with (a) at least one carboxylic acid-reducing enzyme entrapped in a polymer, and (b) at least one carbonyl-reducing composition under conditions wherein the BPA-COOH is degraded.

### Claim 16 (depends on 15)

The method of claim 15, wherein at least one carboxylic acid-reducing enzyme comprises one or more of carboxylate reductase, aldehyde ferredoxin oxidoreductase, formaldehyde ferredoxin oxidoreductase, glyceraldehyde-3-phosphate ferredoxin oxidoreductase, and (2R)-hydroxycarboxylate-viologen-oxidoreductase.

### Claim 17 (depends on 15)

The method of claim 15, wherein the carbonyl-reducing composition is one or more of sodium borohydride, lithium aminoborohydride, and alcohol dehydrogenase.

### Claim 18 (depends on 15)

The method of claim 15, wherein the carboxylic acid reductase and the carbonyl-reducing composition are contacted with the water sequentially.

### Claim 19 (depends on 15)

The method of claim 15, wherein the polymer is organic silane.

### Claim 20 (depends on 19)

The method of claim 19, wherein the organic silane is formed from polymerization of methyltrimethoxysilane and gamma-aminopropyltriethoxysilane.

### Claim 21

A water treatment system that contains 2,2-bis(4-hydroxyphenyl)propanoic acid (BPA-COOH), comprising:
(a) an inlet to a treatment area, wherein the treatment area holds (i) a carboxylic acid-reducing composition comprising at least one carboxylic acid-reducing enzyme entrapped in polymerized organic silane, and (ii) at least one carbonyl-reducing composition; and
(b) an outlet from the treatment area.

### Claim 22 (depends on 21)

The water treatment system of claim 21, wherein the organic silane is formed from polymerization of methyltrimethoxysilane and gamma-aminopropyltriethoxysilane.

### Claim 23 (depends on 21)

The water treatment system of claim 21, wherein at least one carboxylic acid-reducing enzyme comprises one or more of carboxylate reductase, aldehyde ferredoxin oxidoreductase, formaldehyde ferredoxin oxidoreductase, glyceraldehyde-3-phosphate ferredoxin oxidoreductase, and (2R)-hydroxycarboxylate-viologen-oxidoreductase.

### Claim 24 (depends on 21)

The water treatment system of claim 21, wherein the at least one carbonyl-reducing compound is one or more of sodium borohydride, lithium aminoborohydride, and alcohol dehydrogenase.

### Claim 25 (depends on 21)

The water treatment system of claim 21, wherein the carboxylic acid-reducing composition is in the form of one or more of a non-perforated sheet, a perforated sheet, or beads.

### Claim 26 (depends on 21)

The water treatment system of claim 21, wherein the treatment area further comprises a first chamber that holds the carboxylic acid-reducing composition and a second chamber that holds the carbonyl-reducing composition.