# US 2014/0196730 A1 · Tobacco inbred plants k326 src, cms k326 src, k346 src, cms k346 src, nc1562-1 src, nctg-61 src, cms nctg-61 src and hybrid nc196 src

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- Number: US 2014/0196730 A1
- Document ID: US20140196730A1
- Status: Published application
- Kind: A1
- Application number: 14/152,660
- Filed: 2014-01-10
- Published: 2014-07-17
- Earliest claimed: 2013-01-11
- Assignees: North Carolina State University
- Applicants: North Carolina State University
- Inventors: Ramsey S. Lewis; Ralph E. Dewey
- Web page: https://patentgrep.com/patent/US20140196730A1

## CPC

- A01H 6/823: {Nicotiana, e.g. tobacco}
- A01H 5/12: Leaves
- A24B 15/10: Chemical features of tobacco products or tobacco substitutes
- C12N 9/0077: {with a reduced iron-sulfur protein as one donor (1.14.15)}
- C12N 9/1007: {Methyltransferases (general) (2.1.1.)}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A24B: MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present disclosure provides tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC and hybrid NC196 SRC. The present disclosure also provides parts of such plants and products made from those parts. The present disclosure also includes progeny of the provided plants including hybrids.

## Claims

### Claim 1

Cured tobacco material or a tobacco product prepared therefrom, wherein said cured tobacco material is from a tobacco plant, or part thereof, of a hybrid, a cultivar, or a progeny hybrid produced from said cultivar,
said hybrid is NC196 SRC,
said cultivar is selected from the group consisting of K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, and CMS NCTG-61 SRC;
a representative sample seed of said K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NC196 SRC, NCTG-61 SRC, and CMS NCTG-61 SRC having been deposited with the ATCC under ATCC Accession Nos. PTA-13455, PTA-13454, PTA-13453, PTA-13452, PTA-13457, PTA-13458, PTA-120314, and PTA-13456, respectively.

### Claim 2 (depends on 1)

The tobacco product of claim 1, wherein said tobacco product is selected from the group consisting of a pipe tobacco, a cigar tobacco, a cigarette tobacco, a chewing tobacco, a leaf tobacco, a shredded tobacco, and a cut tobacco.

### Claim 3 (depends on 1)

The tobacco product of claim 1, wherein said tobacco product is selected from the group consisting of a cigarillo, a non-ventilated recess filter cigarette, a vented recess filter cigarette, a cigar, snuff, and a chewing tobacco.

### Claim 4 (depends on 3)

The tobacco product of claim 3, wherein said tobacco product has an amount of nornicotine of less than about 3 mg/g.

### Claim 5 (depends on 4)

The tobacco product of claim 4, wherein said amount of nicotine is selected from the group consisting of 3.0 mg/g, 2.5 mg/g, 2.0 mg/g, 1.5 mg/g, 1.0 mg/g, 750 μg/g, 500 pg/g, 250 pg/g, 100 pg/g, 75 pg/g, 50 pg/g, 25 pg/g, 10 pg/g, 7.0 pg/g, 5.0 pg/g, 4.0 pg/g, 2.0 pg/g, 1.0 pg/g, 0.5 pg/g, 0.4 pg/g, 0.2 pg/g, 0.1 pg/g, 0.05 pg/g, 0.01 pg/g, and undetectable.

### Claim 6 (depends on 3)

The tobacco product of claim 3, wherein said tobacco product has an amount of N′-nitrosonornicotine (NNN) of less than about 10 pg/g.

### Claim 7 (depends on 6)

The tobacco product of claim 6, wherein said amount of NNN is selected from the group consisting of 10 pg/g, 7.0 pg/g, 5.0 pg/g, 4.0 pg/g, 2.0 pg/g, 1.0 pg/g, 0.5 pg/g, 0.4 pg/g, 0.2 pg/g, 0.1 pg/g, 0.05 pg/g, 0.01 pg/g, and undetectable.

### Claim 8 (depends on 1)

The tobacco product of claim 1, wherein said cured tobacco material is at least about 5% by dry weight of the total tobacco material in said tobacco product.

### Claim 9 (depends on 8)

The tobacco product of claim 8, wherein said cured tobacco material has a dry weight percentage of said total tobacco material selected from the group consisting of at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, and at least about 95%.

### Claim 10

A method of producing a tobacco product, comprising:
providing cured tobacco material from a tobacco plant, or part thereof, of a hybrid, a cultivar, or a hybrid produced from said cultivar, wherein said hybrid is NC196 SRC, said cultivar is selected from the group consisting of CMS K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, and CMS NCTG-61
preparing a tobacco product from said cured tobacco material,
a representative sample seed of said K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NC196 SRC, NCTG-61 SRC, and CMS NCTG-61 SRC cultivars having been deposited with the ATCC under ATCC Accession Nos. PTA-13455, PTA-13454, PTA-13453, PTA-13452, PTA-13457, PTA-13458, PTA-120314, and PTA-13456, respectively.

### Claim 11

A seed of tobacco cultivar selected from the group consisting of K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NC196 SRC, and CMS NCTG-61 SRC;
a representative sample seed of said K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NC196 SRC, NCTG-61 SRC, and CMS NCTG-61 SRC tobacco cultivars having been deposited with the ATCC under ATCC Accession Nos. PTA-13455, PTA-13454, PTA-13453, PTA-13452, PTA-13457, PTA-13458, PTA-120314, and PTA-13456, respectively.

### Claim 12 (depends on 11)

A tobacco plant, or part thereof, produced from said seed of claim 11.

### Claim 13 (depends on 12)

The part of the plant of claim 12, wherein said part is selected from the group consisting of a harvested leaf, a pollen, an ovule, an embryo, a cotyledon, a hypocotyl, a meristematic cell, a protoplast, a root, a root tip, a pistil, an anther, a flower, a shoot, a stem, a pod, a petiole, and combinations thereof.

### Claim 14 (depends on 12)

A harvested leaf of said tobacco plant of claim 12.

### Claim 15 (depends on 14)

The harvested leaf of claim 14, wherein said leaf has a reduced amount of nornicotine and/or N′-nitrosonomicotine (NNN).

### Claim 16 (depends on 15)

The harvested leaf of claim 15, wherein said reduced amount of nornicotine and/or N-nitrosonornicotine (NNN) is reduced in a smoke stream produced from said leaf.

### Claim 17

An F1 progeny plant of a tobacco cultivar selected from the group consisting of K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, and CMS NCTG-61 SRC;
a representative sample seed of said K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, and CMS NCTG-61 SRC tobacco cultivars having been deposited with the ATCC under ATCC Accession Nos. PTA-13455, PTA-13454, PTA-13453, PTA-13452, PTA-13457, PTA-120314, and PTA-13456, respectively.

### Claim 18 (depends on 17)

The F1 progeny plant of claim 17, wherein said F1 plant is male sterile.