# US 2014/0196631 A1 · Visual assays for coatings incorporating bioactive enzymes for catalytic functions

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- Number: US 2014/0196631 A1
- Document ID: US20140196631A1
- Status: Published application
- Kind: A1
- Application number: 14/156,007
- Filed: 2014-01-15
- Published: 2014-07-17
- Earliest claimed: 2002-09-09
- Assignees: Reactive Surface, Ltd.
- Applicants: Reactive Surface, Ltd.
- Inventors: C. Steven McDaniel
- Web page: https://patentgrep.com/patent/US20140196631A1

## CPC

- C09D 7/65: macromolecular (C09D7/41-C09D7/48 take precedence)
- A01N 63/50: Isolated enzymes; Isolated proteins (peptides A01N37/46)
- C09D 5/14: Paints containing biocides, e.g. fungicides, insecticides or pesticides (C09D5/16 takes precedence)
- C09D 5/1637: {Macromolecular compounds}
- C09D 189/00: Coating compositions based on proteins; Coating compositions based on derivatives thereof
- C12Q 1/34: involving hydrolase
- C12Q 1/44: involving esterase
- C12Q 1/61: involving triglycerides
- C08L 89/00: Compositions of proteins; Compositions of derivatives thereof
- C09D: COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR (cosmetics A61K; processes for applying liquids or other fluent materials to surfaces, in general, B05D; staining wood B27K5/02; glazes or vitreous enamels C03C; natural resins, French polish, drying-oils, driers, turpentine, per se, C09F; polishing compositions other than French polish, ski waxes C09G; adhesives or use of materials as adhesives C09J; materials for sealing or packing joints or covers C09K3/10; materials for stopping leaks C09K3/12; processes for the electrolytic or electrophoretic production of coatings C25D)
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C08L: COMPOSITIONS OF MACROMOLECULAR COMPOUNDS (compositions based on polymerisable monomers C08F, C08G; artificial filaments or fibres D01F; textile treating compositions D06)

## Abstract

Disclosed herein are materials such as a coating, comprising a lipolytic enzyme or organophosphrous compound degrading enzyme. Also disclosed herein are methods of visually detecting enzyme activity in a coating by contacting the coating with a substrate of an enzyme and a visual indicator that changes appearance upon production of a product of enzyme activity on a tack-free coating surface.

## Claims

### Claim at position 1

1-20. (canceled)

### Claim 21

A composition for promoting lipid removal comprising:
a coating material; and
a lipase capable of degrading a lipid component, said lipase associated with said coating material so as to be capable of promoting lipid removal when said coating material is contacted by a lipid.

### Claim 22

A composition for promoting surface contaminant removal comprising:
a coating material; and
a lipase capable of degrading a surface contaminant component, said lipase associated with said coating material so as to be capable of promoting surface contaminant removal when said coating material is contacted by a surface contaminant.

### Claim 23

A composition for promoting lipid removal comprising:
a surface; and
a lipase capable of degrading a lipid component, said lipase associated with said surface so as to be capable of degrading a lipid contacting said surface; and
a lipid, said lipid contacting said surface and said lipase.

### Claim 24

A composition for promoting surface contaminant removal comprising:
a surface; and
a lipase capable of degrading a surface contaminant component, said lipase associated with said surface so as to be capable of degrading a surface contaminant contacting said surface; and
a surface contaminant, said surface contaminant contacting said surface and said lipase.

### Claim 25

A curable composition for promoting lipid removal comprising:
a lipase; and
a solvent-borne two-component polyurethane coating material;
said lipase dispersed into said coating by covalent interaction with said coating material so as to be capable of degrading a lipid when said coating is contacted by a lipid.

### Claim 26

A curable composition for promoting surface contaminant removal comprising:
a lipase; and
a solvent-borne two-component polyurethane coating material;
said lipase dispersed into said coating by covalent interaction with said coating material so as to be capable of degrading a surface contaminant when said coating is contacted by a surface contaminant.