# US 2014/0194690 A1 · Chemically-selective, label free, microendoscopic system based on coherent anti-stokes raman scattering

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- Number: US 2014/0194690 A1
- Document ID: US20140194690A1
- Status: Published application
- Kind: A1
- Application number: 13/843,886
- Filed: 2013-03-15
- Published: 2014-07-10
- Earliest claimed: 2010-07-08
- Assignees: The Methodist Hospital Research Institute
- Inventors: Stephen T.C. Wong; Zhiyong Wang; Ganesh Palpattu
- Web page: https://patentgrep.com/patent/US20140194690A1

## CPC

- A61B 1/00165: {with light-conductive means, e.g. fibre optics (A61B1/07 takes precedence)}
- A61B 1/000094: {extracting biological structures}
- A61B 1/00172: {with means for scanning}
- A61B 1/063: {for monochromatic or narrow-band illumination}
- A61B 1/07: using light-conductive means, e.g. optical fibres
- A61B 5/0066: {Optical coherence imaging}
- A61B 5/0075: {by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy (A61B5/0071 takes precedence)}
- A61B 5/0084: {for introduction into the body, e.g. by catheters (A61B5/1459 takes precedence)}
- A61B 5/6847: {mounted on an invasive device}
- A61B 18/18: by applying electromagnetic radiation, e.g. microwaves
- G01N 21/65: Raman scattering
- G06T 7/30: Determination of transform parameters for the alignment of images, i.e. image registration
- G06V 20/693: {Acquisition}
- A61B 5/7207: {of noise induced by motion artifacts}
- A61B 6/12: Arrangements for detecting or locating foreign bodies
- A61B 6/486: {involving generating temporal series of image data}
- G01N 2021/653: {Coherent methods [CARS]}
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- G06T: IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06V: IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING

## Abstract

An endoscopic microscopic system for collecting and processing a sequence of images.

## Claims

### Claim at position 1

1-65. (canceled)

### Claim 66

A method of calculating a global registration for one or more images comprising: calculating the global registration for one or more of the images by iteratively minimizing an energy function that is a function of normalized mutual information.

### Claim at position 3

67-70. (canceled)

### Claim 71

A method of calculating a global registration for one or more images comprising: estimating motion within one or more of the images using line by line searching; dividing one or more of the images into resting and movement time periods; and using a speed embedded hidden markov model for motion correction of one or more of the images.

### Claim at position 5

72-80. (canceled)

### Claim 81

A method of calculating a global registration for one or more images comprising: preprocessing one or more of the images; training a motion estimating model for one or more of the images; and estimating a motion correction model for one or more of the images.

### Claim at position 7

82-84. (canceled)

### Claim 85

A CARS microscopy system, comprising: a source of a Stokes laser beam; a source of a pump laser beam; an optical fiber operably coupled to the sources of the Stokes and pump laser beams for conveying the Stokes and pump laser beams; a long pass filter operably coupled to the optical fiber; and one or more optical detectors operably coupled to the optical fiber for detecting CARS signals; wherein the optical fiber comprises a multimode fiber.

### Claim 86 (depends on 85)

The system of claim 85, wherein a portion of the optical fiber comprises a single mode fiber.

### Claim 87 (depends on 85)

The system of claim 85, wherein the optical fiber comprises a multimode fiber.

### Claim 88 (depends on 85)

The system of claim 85, wherein the optical fiber comprises a single mode fiber and a multimode fiber.

### Claim at position 12

89-95. (canceled)

### Claim 96 (depends on 85)

The system of claim 85, further comprising a polarization controller operably coupled to the optical fiber.

### Claim 97 (depends on 96)

The system of claim 96, wherein the polarization controller is adapted to modify a polarization of a signal relative to the polarization of another signal.

### Claim 98 (depends on 85)

The system of claim 85, wherein the polarization of the Stokes laser beam and the pump laser beam are orthogonal to one another.

### Claim 99 (depends on 85)

The system of claim 85, further comprising a polarization controller operably coupled to the sources of the Stokes laser beam and the pump laser beam for modifying a polarization of at least one of the Stokes laser beam and the pump laser beam.

### Claim 100 (depends on 99)

The system of claim 99, further comprising a polarization controller operably coupled to the optical fiber.

### Claim 101 (depends on 100)

The system of claim 100, wherein the polarization controller is adapted to modify a polarization of a signal relative to the polarization of another signal.

### Claim 102 (depends on 99)

The system of claim 99, wherein the polarization of the Stokes laser beam and the pump laser beam are orthogonal to one another.

### Claim 103 (depends on 85)

The system of claim 85, further comprising a polarization controller operably coupled to the optical fiber.

### Claim 104 (depends on 103)

The system of claim 103, wherein the polarization of the Stokes laser beam and the pump laser beam are not equal.

### Claim 105 (depends on 104)

The system of claim 104, wherein the polarization controller is adapted to modify a polarization of a signal relative to the polarization of another signal.

### Claim at position 23

106-111. (canceled)

### Claim 112

A method of reducing four wave mixing within a CARS microscopy system including a source of a Stokes laser beam, a source of a pump laser beam, an optical fiber operably coupled to the sources of the Stokes and pump laser beams for conveying the Stokes and pump laser beams, and one or more optical detectors operably coupled to the optical fiber for detecting CARS signals, comprising: modifying a polarization of the Stokes laser beam with respect to the pump laser beam prior to passing the Stokes and pump laser beams in a forward direction through the optical fiber such that their polarizations are not identical.

### Claim at position 25

113-118. (canceled)

### Claim 119

A system for reducing four wave mixing within a CARS microscopy system including a source of a Stokes laser beam, a source of a pump laser beam, an optical fiber operably coupled to the sources of the Stokes and pump laser beams for conveying the Stokes and pump laser beams, and one or more optical detectors operably coupled to the optical fiber for detecting CARS signals, comprising: means for modifying a polarization of the Stokes laser beam with respect to the pump laser beam prior to passing the Stokes and pump laser beams in a forward direction through the optical fiber such that their polarizations are not identical.

### Claim at position 27

120-125. (canceled)

### Claim 126

A method for diagnosing and treating a patient, comprising: obtaining one or more CARS images of tissue within the patient; and as a function of one or more attributes of the CARS images, determining the type of tissue.

### Claim at position 29

127-167. (canceled)