# US 2014/0194609 A1 · Isolation of nucleic acids

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- Number: US 2014/0194609 A1
- Document ID: US20140194609A1
- Status: Published application
- Kind: A1
- Application number: 14/145,087
- Filed: 2013-12-31
- Published: 2014-07-10
- Earliest claimed: 2011-05-11
- Assignees: Exact Sciences Corporation
- Applicants: Exact Sciences Corporation
- Inventors: Michael J. Domanico; James P. Light, II; Keith Kopitzke; John Zeis
- Web page: https://patentgrep.com/patent/US20140194609A1

## CPC

- C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence)
- B01D 33/15: with rotary plane filtering surfaces
- B01D 33/155: {the filtering surface being parallel to the rotation axis}
- B01L 3/00: Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L 3/5021: {Test tubes specially adapted for centrifugation purposes}
- B03C 1/30: Combinations with other devices, not otherwise provided for
- B04B 3/00: Centrifuges with rotary bowls in which solid particles or bodies become separated by centrifugal force and simultaneous sifting or filtering
- C12N 15/1006: {by means of a solid support carrier, e.g. particles, polymers}
- C12N 15/1013: {by using magnetic beads}
- C12N 15/1017: {by filtration, e.g. using filters, frits, membranes}
- C12Q 1/6886: for cancer (immunoassay for cancer G01N33/575)
- C12Q 2600/158: Expression markers
- C12Q 2600/16: Primer sets for multiplex assays
- Y10T 436/143333: Saccharide [e.g., DNA, etc.]
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B03C: MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS (separating isotopes B01D59/00; combinations of magnetic or electrostatic separation with separation of solids by other means B03B, B07B)
- B04B: CENTRIFUGES (high-speed drum mills B02C19/11; domestic spin driers D06F; analysing, measuring or monitoring physical or chemical properties of samples during centrifuging, see the relevant subclasses for these procedures, e.g. G01N)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.

## Claims

### Claim 1

A method of producing a filtrate from a sample, the method comprising:
a) placing a sample to be filtered into a spin filter, said spin filter comprising
i) a hollow body;
ii) a bottom end; and
iii) an open top end opposite the bottom end,
said hollow body made from a porous filtering material; and
b) centrifuging said spin filter,
wherein during said centrifuging, a fraction of said sample passes through said porous filtering material of said spin filter to produce a filtrate.

### Claim 2 (depends on 1)

The method of claim 1 wherein the bottom end of said spin filter is made from a porous filtering material.

### Claim 3 (depends on 1)

The method of claim 1 wherein the porous filtering material of said spin filter is polyethylene.

### Claim 4 (depends on 1)

The method of claim 1 wherein the porous filtering material of said spin filter has a nominal pore size of 20 micrometers.

### Claim 5 (depends on 1)

The method of claim 1 wherein the bottom end of said spin filter has a shape selected from the group consisting of a hemisphere, a disc, a cone, or a portion of an ellipsoid.

### Claim 6 (depends on 1)

A method of claim 1, wherein said spin filter is placed in a collection vessel adapted to receive the spin filter, and wherein during said centrifuging, said filtrate is retained in said collection vessel.

### Claim 7 (depends on 1)

A kit comprising a spin filter of claim 1 and an instruction for use.

### Claim 8 (depends on 7)

The kit of claim 7, further comprising a collection vessel.