# US 2014/0194543 A1 · Chromatographic media

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- Number: US 2014/0194543 A1
- Document ID: US20140194543A1
- Status: Published application
- Kind: A1
- Application number: 14/066,251
- Filed: 2013-10-29
- Published: 2014-07-10
- Earliest claimed: 2005-01-25
- Inventors: Nandu Deorkar; Robert C. Buss; Joseph M. Mladosich; Paul A. Bouis
- Web page: https://patentgrep.com/patent/US20140194543A1

## CPC

- B01J 41/14: obtained by reactions only involving unsaturated carbon-to-carbon bonds
- B01D 15/327: {with hydrophobic interaction}
- B01D 15/363: {Anion-exchange}
- B01D 15/364: {Amphoteric or zwitterionic ion-exchanger}
- B01J 20/261: {obtained by reactions only involving carbon to carbon unsaturated bonds (macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F)}
- B01J 20/262: {obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation (macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds per se C08G)}
- B01J 20/265: {modified or post-treated polymers (polymer carriers or substrates subjected to further impregnating or coating B01J20/3208)}
- B01J 20/28004: {Sorbent size or size distribution, e.g. particle size}
- B01J 20/285: based on polymers
- B01J 20/288: Polar phases
- B01J 20/3092: {Packing of a container, e.g. packing a cartridge or column (of chromatography columns B01D15/206)}
- B01J 20/321: {consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds}
- B01J 20/3255: {comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. heterocyclic or heteroaromatic structures}
- B01J 20/3272: {Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds}
- B01J 20/328: {Polymers on the carrier being further modified}
- B01J 20/3285: {Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic}
- C07K 1/165: {mixed-mode chromatography}
- B01J 2220/52: Sorbents specially adapted for preparative chromatography
- B01J 2220/54: Sorbents specially adapted for analytical or investigative chromatography
- B01J 2220/58: Use in a single column
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

This invention concerns the preparation and use of novel polymeric chromatographic media and preferably mixed mode polymeric chromatographic media. In accordance with the present invention, polymeric media is prepared using polymeric particles derivatized with polyethyleneimine, and preferably such polyethyleneimine derivatized polymeric particles further functionalized with appropriate reactants. The polymeric chromatographic media is especially useful for bioseparations.

## Claims

### Claim 1

Chromatographic media comprising polymeric resin particles derivatized with polyethyleneimine on the surface of the polymer.

### Claim 2 (depends on 1)

Chromatographic media according to claim 1 wherein the polymeric particles comprise polymers selected from the group consisting of cellulose, agarose, epoxidized or halogenated polystyrenes, epoxidized or halogenated polyacrylates or polymethacrylates, and epoxidized or halogenated polydivinylbenzenes.

### Claim 3 (depends on 2)

Chromatographic media according to claim 2 wherein the polymeric particles comprise polymers selected from the group consisting of epoxidized or halogenated polystyrenes, epoxidized or halogenated polyacrylates or polymethacrylates, and epoxidized or halogenated polydivinylbenzenes.

### Claim 4 (depends on 1)

Chromatographic media according to claim 1 wherein the polymeric resin particles derivatized with polyethyleneimine on the surface of the polymer are functionalized by reaction of a functionalization reagent with terminal amino groups of the polyethyneneimine on the surface of the polymeric resin.

### Claim 5 (depends on 3)

Chromatographic media according to claim 3 wherein the polymeric resin particles derivatized with polyethyleneimine on the surface of the polymer are functionalized by reaction of a functionalization reagent with terminal amino groups of the polyethyneneimine on the surface of the polymeric resin.

### Claim 6 (depends on 4)

Chromatographic media according to claim 4 wherein the functionalization agent is selected from the group consisting of: acid anhydrides, sulfonation agents, alkyl chlorides, and alkyl chlorides containing quaternary ammonium functionality, and mixtures thereof.

### Claim 7 (depends on 6)

Chromatographic media according to claim 6 wherein the functionalization reagent is selected from the group consisting of cyclic carboxylic anhydrides, unsaturated carboxylic anhydrides, bisulfites, alkyl chlorides, alkyl anhydrides, alkyl chlorides containing quaternary ammonium functionality and mixtures thereof

### Claim 8 (depends on 7)

Chromatographic media according to claim 7 wherein the functionalization reagent is selected from the group consisting of: glutaric anhydride, succinic anhydrides, maleic anhydride, sodium meta-bisulfite, burtryl chloride, acetic anhydride, butyric anhydride, (3-chloro-2-hydroxypropyl)trimethylammonium chloride, and mixtures thereof.

### Claim 9 (depends on 1)

Chromatographic media according to claim 1 in which is the media is mixed mode.

### Claim 10 (depends on 4)

Chromatographic media according to claim 4 in which is the media is mixed mode.

### Claim 11 (depends on 10)

Chromatographic media according to claim 10 wherein the mixed mode media is selected from the group consisting of media with mixed primary, secondary and tertiary amine exchange sites, media with both weak anion and weak cation exchange sites, media with weak anion, weak cation and strong cation exchange sites, media with weak anion and hydrophobic (reverse phase) exchange sites, and media with weak anion and strong anion.

### Claim 12 (depends on 1)

A column for chromatography which is packed with chromatographic media according to claim 1.

### Claim 13 (depends on 3)

A column for chromatography which is packed with chromatographic media according to claim 3.

### Claim 14 (depends on 5)

A column for chromatography which is packed with chromatographic media according to claim 5.

### Claim 15 (depends on 8)

A column for chromatography which is packed with chromatographic media according to claim 8.

### Claim 16 (depends on 12)

A process for separation of components of a solution comprising passing the solution through a chromatography column of claim 12 and eluting components of the solution.

### Claim 17 (depends on 13)

A process for separation of components of a solution comprising passing the solution through a chromatography column of claim 13 and eluting components of the solution.

### Claim 18 (depends on 14)

A process for separation of components of a solution comprising passing the solution through a chromatography column of claim 14 and eluting components of the solution.

### Claim 19 (depends on 15)

A process for separation of components of a solution comprising passing the solution through a chromatography column of claim 15 and eluting components of the solution.

### Claim 20 (depends on 17)

A process according to claim 17 wherein the solution is a solution containing proteins.