# US 2014/0194335 A1 · Compositions and methods for treating biofilms > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0194335 A1 - Document ID: US20140194335A1 - Status: Published application - Kind: A1 - Application number: 14/241,217 - Filed: 2012-08-26 - Published: 2014-07-10 - Earliest claimed: 2011-08-26 - Assignees: Ohio University - Inventors: Tingyue Gu; Dake Xu - Web page: https://patentgrep.com/patent/US20140194335A1 ## CPC - C11D 3/48: Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions - C02F 1/50: by addition or application of a germicide or by oligodynamic treatment {(C02F1/4606, C02F1/467, C02F1/76 take precedence)} - C02F 1/76: with halogens or compounds of halogens {(C02F1/4674 takes precedence)} - C02F 1/766: {by means of halogens other than chlorine or of halogenated compounds containing halogen other than chlorine} - C02F 2303/08: Corrosion inhibition - C02F 2303/20: Prevention of biofouling - C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL - C02F: TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE (separation in general B01D; special arrangements on waterborne vessels of installations for treating water, waste water or sewage, e.g. for producing fresh water, B63J; adding materials to water to prevent corrosion C23F; treating radioactively-contaminated liquids G21F9/04; regeneration of reactants for recirculation into processes, see the relevant places for the processes) ## Abstract Compositions and methods for treating biofilm formation and growth on a substrate are provided. The composition comprises 1 ppb to 1,000 ppm of at least one D-amino acid and 1 ppm to 60,000 ppm of at least one biocide. The method comprises contacting the substrate with 1 ppb to 1,000 ppm of at least one D-amino acid and 1 ppm to 60,000 ppm of at least one biocide. The compositions and methods are effective for preventing, reducing or eliminating biofilm formation or biofilm growth or both, as well as eradicating established, recalcitrant biofilms, particularly biofilms comprising sulfate reducing bacteria that are known to cause microbiologically influenced corrosion, biofouling, or both. ## Claims ### Claim 1 A composition for treating biofilm formation and growth on a substrate, the composition comprising: 1 ppb to 1,000 ppm of at least one D-amino acid; and 1 ppm to 60,000 ppm of at least one biocide. ### Claim 2 (depends on 1) The composition according to claim 1, wherein the D-amino acid is selected from the group consisting of D-tyrosine, D-methionine, D-tryptophan, D-leucine, D-arginine, D-histidine, D-lysine, D-aspartic acid, D-glutamic acid, D-serine, D-threonine, D-asparagine, D-glutamine, D-cysteine, D-proline, D-alanine, D-valine, D-isoleucine, D-phenylalanine, a non-standard D-amino acid, and combinations thereof. ### Claim 3 (depends on 1) The composition according to claim 1, wherein the D-amino acid is selected from the group consisting of D-tyrosine, D-methionine, D-tryptophan, D-leucine, and combinations thereof. ### Claim 4 (depends on 1) The composition according to claim 1, wherein the D-amino acid is D-tyrosine. ### Claim 5 (depends on 1) The composition according to claim 1, wherein the biocide is selected from the group consisting of tetrakis hydroxymethyl phosphonium sulfate (THPS), glutaraldehyde, chlorine monoxide, chlorine dioxide, calcium hypochlorite, potassium hypochlorite, sodium hypochlorite, dibromonitriloproprionamide (DBNPA), methylene bis(thiocyanate) (MBT), 2-(thiocyanomethylthio)benzothiazole (TCMTB), bronopol, 2-bromo-2-nitro-1,3-propanediol (BNPD), tributyl tetradecyl phosphonium chloride (TTPC), taurinamide and derivatives thereof, phenols, quaternary ammonium salts, chlorine-containing agents, quinaldinium salts, lactones, organic dyes, thiosemicarbazones, quinones, carbamates, urea, salicylamide, carbanilide, guanide, amidines, imidazolines, acetic acid, benzoic acid, sorbic acid, propionic acid, boric acid, dehydroacetic acid, sulfurous acid, vanillic acid, p-hydroxybenzoate esters, isopropanol, propylene glycol, benzyl alcohol, chlorobutanol, phenylethyl alcohol, formaldehyde, iodine and solutions thereof, povidone-iodine, hexamethylenetetramine, noxythiolin, 1-(3-chloroallyl)-3,5,7-triazo-1-azoniaadamantane chloride, taurolidine, taurultam, N-(5-nitro-2-furfurylidene)-1-amino-hydantoin, 5-nitro-2-furaldehyde semicarbazone, 3,4,4′-trichlorocarbanilide, 3,4′,5-tribromosalicylanilide, 3-trifluoromethyl-4,4′-dichlorocarbanilide, 8-hydroxyquinoline, 1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid, 1,4-dihydro-1-ethyl-6-fluoro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid, hydrogen peroxide, peracetic acid, sodium oxychlorosene, parachlorometaxylenol, 2,4,4′-trichloro-2′-hydroxydiphenol, thymol, chlorhexidine, benzalkonium chloride, cetylpyridinium chloride, silver sulfadiazine, silver nitrate, bromine, ozone, isothiazolones, polyoxyethylene (dimethylimino) ethylene (dimethylimino) ethylene dichloride, 2-(tert-butylamino)-4-chloro-6-ethylamino-5-triazine (terbutylazine), and combinations thereof. ### Claim 6 (depends on 1) The composition according to claim 1, wherein the biocide is tetrakis hydroxymethyl phosphonium sulfate (THPS). ### Claim 7 A method for treating biofilm formation and growth on a substrate, the method comprising: contacting the substrate with 1 ppb to 1,000 ppm of at least one D-amino acid and 1 ppm to 60,000 ppm of at least one biocide. ### Claim 8 (depends on 7) The method according to claim 7, wherein the substrate is one of a metal, a metal alloy, nylon, plastic, composite material, wood, glass, ceramic, porcelain, a painted surface, rock, or soil. ### Claim 9 (depends on 7) The method according to claim 7, wherein the substrate is selected from the group consisting of a pipe, a holding vessel, underground rock formations, underground soil formations, a ship hull, a wellbore, infrastructure, a beam, a trough, a girder, sheeting, prefabricated structures, and underwater structures. ### Claim 10 (depends on 7) The method according to claim 7, wherein the substrate is a pipe. ### Claim 11 (depends on 7) The method according to claim 7, wherein the at least one D-amino acid and the at least one biocide are separately and simultaneously added to a medium that contacts the substrate. ### Claim 12 (depends on 7) The method according to claim 7, wherein the at least one D-amino acid and the at least one biocide are in a solution that is added to a medium that contacts the substrate. ### Claim 13 (depends on 7) The method according to claim 7, wherein the at least one D-amino acid and the at least one biocide are separately and sequentially added to a medium that contacts the substrate. ### Claim 14 (depends on 11) The method according to claim 11, wherein the medium is selected from the group consisting of an oil, an aqueous solution, a hydraulic fracturing fluid, a fuel, carbon dioxide, a natural gas, an oil/water mixture, a fuel/water mixture, water containing salts, ocean or seawater, brackish water, sources of freshwater, lakes, rivers, stream, bogs, ponds, marshes, runoff from the thawing of snow or ice, springs, groundwater, aquifers, precipitation, any substance that is a liquid at ambient temperature and is hydrophobic but soluble in organic solvents, hexanes, benzene, toluene, chloroform, diethyl ether, vegetable oils, petrochemical oils, crude oil, refined petrochemical products, volatile essential oils, fossil fuels, gasoline, mixtures of hydrocarbons, jet and rocket fuels, biofuels, and combinations thereof. ### Claim 15 (depends on 11) The method according to claim 11, wherein the medium is an oil/water mixture. ### Claim 16 (depends on 7) The method according to claim 7, wherein the D-amino acid is selected from the group consisting of D-tyrosine, D-methionine, D-tryptophan, D-leucine, D-arginine, D-histidine, D-lysine, D-aspartic acid, D-glutamic acid, D-serine, D-threonine, D-asparagine, D-glutamine, D-cysteine, D-proline, D-alanine, D-valine, D-isoleucine, D-phenylalanine, a non-standard D-amino acid, and combinations thereof. ### Claim 17 (depends on 7) The method according to claim 7, wherein the D-amino acid is selected from the group consisting of D-tyrosine, D-methionine, D-tryptophan, D-leucine, and combinations thereof. ### Claim 18 (depends on 7) The method according to claim 7, wherein the biocide is selected from the group consisting of tetrakis hydroxymethyl phosphonium sulfate (THPS), glutaraldehyde, chlorine monoxide, chlorine dioxide, calcium hypochlorite, potassium hypochlorite, sodium hypochlorite, dibromonitriloproprionamide (DBNPA), methylene bis(thiocyanate) (MBT), 2-(thiocyanomethylthio)benzothiazole (TCMTB), bronopol, 2-bromo-2-nitro-1,3-propanediol (BNPD), tributyl tetradecyl phosphonium chloride (TTPC), taurinamide and derivatives thereof, phenols, quaternary ammonium salts, chlorine-containing agents, quinaldinium salts, lactones, organic dyes, thiosemicarbazones, quinones, carbamates, urea, salicylamide, carbanilide, guanide, amidines, imidazolines, acetic acid, benzoic acid, sorbic acid, propionic acid, boric acid, dehydroacetic acid, sulfurous acid, vanillic acid, p-hydroxybenzoate esters, isopropanol, propylene glycol, benzyl alcohol, chlorobutanol, phenylethyl alcohol, formaldehyde, iodine and solutions thereof, povidone-iodine, hexamethylenetetramine, noxythiolin, 1-(3-chloroallyl)-3,5,7-triazo-1-azoniaadamantane chloride, taurolidine, taurultam, N-(5-nitro-2-furfurylidene)-1-amino-hydantoin, 5-nitro-2-furaldehyde semicarbazone, 3,4,4′-trichlorocarbanilide, 3,4′,5-tribromosalicylanilide, 3-trifluoromethyl-4,4′-dichlorocarbanilide, 8-hydroxyquinoline, 1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid, 1,4-dihydro-1-ethyl-6-fluoro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid, hydrogen peroxide, peracetic acid, sodium oxychlorosene, parachlorometaxylenol, 2,4,4′-trichloro-2′-hydroxydiphenol, thymol, chlorhexidine, benzalkonium chloride, cetylpyridinium chloride, silver sulfadiazine, silver nitrate, bromine, ozone, isothiazolones, polyoxyethylene (dimethylimino) ethylene (dimethylimino) ethylene dichloride, 2-(tert-butylamino)-4-chloro-6-ethylamino-5-triazine (terbuthylazine), and combinations thereof. ### Claim 19 (depends on 7) The method according to claim 7, wherein the biocide is 10 ppm to 1,000 ppm tetrakis hydroxymethyl phosphonium sulfate (THPS), and the D-amino acid is 100 ppb to 100 ppm D-tyrosine. ### Claim 20 (depends on 7) The method according to claim 7, wherein the biocide is 10 ppm to 1,000 ppm tetrakis hydroxymethyl phosphonium sulfate (THPS), and the D-amino acid is a 100 ppb to 100 ppm D-amino acid mixture comprising equimolar amounts of D-tyrosine, D-methionine, D-tryptophan, and D-leucine, and further comprising 100 ppm to 1,000 ppm of a surfactant.