# US 2014/0194319 A1 · Use of microvesicles in diagnosis and prognosis of medical diseases and conditions

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- Number: US 2014/0194319 A1
- Document ID: US20140194319A1
- Status: Published application
- Kind: A1
- Application number: 14/219,399
- Filed: 2014-03-19
- Published: 2014-07-10
- Earliest claimed: 2008-02-01
- Assignees: The General Hospital Corporation
- Applicants: The General Hospital Corporation
- Inventors: Johan Karl Olov SKOG; Xandra O. BREAKEFIELD; Dennis BROWN; Kevin C. MIRANDA; Leileata M. RUSSO
- Web page: https://patentgrep.com/patent/US20140194319A1

## CPC

- C12Q 1/6883: for diseases caused by alterations of genetic material
- A61P 35/00: Antineoplastic agents
- C12N 15/1017: {by filtration, e.g. using filters, frits, membranes}
- C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence)
- C12Q 1/6886: for cancer (immunoassay for cancer G01N33/575)
- C12Q 2600/106: Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
- C12Q 2600/112: Disease subtyping, staging or classification
- C12Q 2600/156: Polymorphic or mutational markers
- C12Q 2600/158: Expression markers
- C12Q 2600/178: miRNA, siRNA or ncRNA
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The presently disclosed subject matter is directed to methods of aiding diagnosis, prognosis, monitoring and evaluation of a disease or other medical condition in a subject by detecting a biomarker in microvesicles isolated from a biological sample from the subject.

## Claims

### Claim 1

A diagnostic method, wherein said method aids in the detection of a disease or other medical condition in a subject, the method comprising the steps of:
(a) isolating a microvesicle fraction from a biological sample from the subject;
(b) detecting the presence or absence of a biomarker within the microvesicle fraction, wherein the biomarker is associated with the disease or other medical condition.