# US 2014/0194287 A1 · Crushed bacterial body and compositions thereof

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- Number: US 2014/0194287 A1
- Document ID: US20140194287A1
- Status: Published application
- Kind: A1
- Application number: 14/126,154
- Filed: 2012-06-14
- Published: 2014-07-10
- Earliest claimed: 2011-06-14
- Assignees: Soma, Gen-Ichiro; BioMedical Research Group Inc.
- Inventors: Gen-Ichiro Soma; Hiroyuki Inagawa; Chie Kohchi
- Web page: https://patentgrep.com/patent/US20140194287A1

## CPC

- A01N 63/20: Bacteria; Substances produced thereby or obtained therefrom
- A23K 10/16: Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions (A23K10/12 takes precedence)
- A23K 20/00: Accessory food factors for animal feeding-stuffs
- A23K 20/10: Organic substances
- A23K 40/10: by agglomeration; by granulation, e.g. making powders
- A23K 50/40: for carnivorous animals, e.g. cats or dogs
- A23L 33/10: using additives (addition of substantially indigestible substances A23L33/21)
- A23L 33/135: Bacteria or derivatives thereof, e.g. probiotics
- A61K 8/99: from microorganisms other than algae or fungi, e.g. protozoa or bacteria
- A61K 35/74: Bacteria (therapeutic use of a bacterial protein A61K38/00)
- A61K 39/39: characterised by the immunostimulating additives, e.g. chemical adjuvants
- A61P 3/00: Drugs for disorders of the metabolism (of the blood or the extracellular fluid A61P7/00)
- A61P 3/02: Nutrients, e.g. vitamins, minerals
- A61P 25/00: Drugs for disorders of the nervous system
- A61P 25/22: Anxiolytics
- A61P 31/00: Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P 35/00: Antineoplastic agents
- A61P 37/02: Immunomodulators
- A61P 37/04: Immunostimulants
- A61P 37/08: Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14)
- A61Q 19/00: Preparations for care of the skin
- C05F 11/00: Other organic fertilisers
- C07K 14/472: {Complement proteins, e.g. anaphylatoxin, C3a, C5a}
- C12N 1/066: by physical processes
- C12N 1/20: Bacteria; Culture media therefor
- A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs
- A61K 2800/10: General cosmetic use
- A61K 2800/70: Biological properties of the composition as a whole
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A23K: FODDER
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C05F: ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION

## Abstract

A crushed bacterial body can be obtained by culturing a gram-negative bacterium and physically crushing a body of the gram-negative bacterium, comprises all components including immunostimulatory components in the bacterial body, and comprises LPSs having molecular weights of 20,000 or less as active ingredients. The crushed bacterial body is effective as a health food, drug, skin-care product, bath agent, feedstuff for protecting crustaceans, shellfish, fish, poultry, farm animals and pet animals from infections, and a feed additive for growth promotion, and can provide an innate immunostimulatory substance derived from a gram-negative bacterial body having infection-preventing effects and growth promoting effects, an inexpensive and practical microbial crushed bacterial body, and a composition thereof.

## Claims

### Claim 1

A crushed bacterial body which can be obtained by culturing a gram-negative bacterium and physically crushing a body of the gram-negative bacterium after the body was heated, comprises all components including immunostimulatory components in the bacterial body after the body was heated, and comprises LPSs having molecular weights of 20,000 or less as active ingredients.

### Claim 2 (depends on 1)

The crushed bacterial body according to claim 1, wherein the gram-negative bacterium is Escherichia coli, Serratia bacterium, Aeromonas bacterium, Rahnella bacterium, Enterobacter bacterium, Xanthomonas bacterium, Zymomonas bacterium, Pantoea bacterium or Acetobacter bacterium.

### Claim 3 (depends on 1)

A composition of the crushed bacterial body contains the crushed bacterial body according to claim 1 wherein said composition is selected from a pharmaceutical, quasi drug, cosmetic, food, functional food, bath agent, feedstuff, pet food or veterinary drug.

### Claim 4 (depends on 1)

A composition of the crushed bacterial body contains the crushed bacterial body according to claim 1 wherein said composition is selected from a fertilizer, a compost or a pharmaceutical for plants.

### Claim 5 (depends on 3)

The composition according to claim 3 which is intended for growth promotion, immune activation, prevention of lifestyle-related diseases, cancers or allergic diseases, infection prevention, or stress resistance.

### Claim 6 (depends on 4)

The composition according to claim 4 which is intended for growth promotion, immune activation, infection prevention or stress resistance.

### Claim 7 (depends on 2)

A composition of the crushed bacterial body contains the crushed bacterial body according to claim 2 wherein said composition is selected from a pharmaceutical, quasi drug, cosmetic, food, functional food, bath agent, feedstuff, pet food or veterinary drug.

### Claim 8 (depends on 2)

A composition of the crushed bacterial body contains the crushed bacterial body according to claim 2 wherein said composition is selected from a fertilizer, a compost or a pharmaceutical for plants.

### Claim 9 (depends on 7)

The composition according to claim 7 which is intended for growth promotion, immune activation, prevention of lifestyle-related diseases, cancers or allergic diseases, infection prevention, or stress resistance.

### Claim 10 (depends on 8)

The composition according to claim 8 which is intended for growth promotion, immune activation, infection prevention or stress resistance.