# US 2014/0193925 A1 · Biosensors > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0193925 A1 - Document ID: US20140193925A1 - Status: Published application - Kind: A1 - Application number: 13/548,521 - Filed: 2012-07-13 - Published: 2014-07-10 - Earliest claimed: 2011-08-11 - Assignees: Massachusetts Institute of Technology - Inventors: Dhiman Bhattacharyya; Karen K. Gleason - Web page: https://patentgrep.com/patent/US20140193925A1 ## CPC - G01N 33/54366: {Apparatus specially adapted for solid-phase testing} - G01N 27/3275: {Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction} - G01N 33/531: Production of immunochemical test materials - G01N 33/54306: {Solid-phase reaction mechanisms} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract A chemiresistive biosensor for detecting an analyte can include a high specific surface area substrate conformally coated with a conductive polymer, and a binding reagent immobilized on the conductive polymer, wherein the binding reagent has a specific affinity for the analyte. The conductive polymer can be deposited on a substrate by oCVD. ## Claims ### Claim 1 A chemiresistive biosensor configured to detect an analyte comprising: a high specific surface area substrate conformally coated with a conductive polymer; and a binding reagent immobilized on the conductive polymer, wherein the binding reagent has a specific affinity for the analyte. ### Claim 2 (depends on 1) The biosensor of claim 1, wherein the substrate includes an electro-spun polymer fiber mat. ### Claim 3 (depends on 1) The biosensor of claim 1, wherein the substrate has a BET surface area of at least 5 m2/g. ### Claim 4 (depends on 1) The biosensor of claim 1, wherein the conductive polymer is a copolymer including the monomer units -[A]-, -[B]-, and -[B*]-; wherein A, B, and B* are distinct monomers; a homopolymer of monomer A is a highly conductive polymer; monomer B includes a reactive functional group selected to form a covalent link to a binding reagent; and monomer B* is monomer B covalently linked to the binding reagent. ### Claim 5 (depends on 4) The biosensor of claim 4, wherein the copolymer has formula (I): -[A]x-[B]y-[B*]z- (I) wherein x, y, and z are the mole fractions of monomer A, B, and B* in the copolymer, respectively; and 0y+z. ### Claim 6 (depends on 4) The biosensor of claim 4, wherein monomer A and monomer B, independently, are each an optionally substituted aniline monomer, an optionally substituted pyrrole monomer, or an optionally substituted thiophene monomer. ### Claim 7 (depends on 4) The biosensor of claim 4, wherein monomer A is an EDOT monomer. ### Claim 8 (depends on 4) The biosensor of claim 4, wherein monomer B is a 3-TE monomer or a TAA monomer. ### Claim 9 (depends on 1) The biosensor of claim 1, wherein the binding reagent is a protein. ### Claim 10 (depends on 1) A sensor array comprising a plurality of sensor elements, wherein each sensor element includes a chemiresistive biosensor according to claim 1, and wherein each sensor element is configured to detect a different analyte. ### Claim 11 A method of detecting an analyte, comprising: contacting a sample suspected of containing the analyte with a chemiresistive biosensor comprising: a high specific surface area substrate conformally coated with a conductive polymer; and a binding reagent immobilized on the conductive polymer; wherein the binding reagent has a specific affinity for the analyte. ### Claim 12 (depends on 11) The method of claim 11, wherein the substrate includes an electro-spun polymer fiber mat. ### Claim 13 (depends on 11) The method of claim 11, wherein the substrate has a BET surface area of at least 5 m2/g. ### Claim 14 (depends on 11) The method of claim 11, wherein the conductive polymer is a copolymer including the monomer units -[A]-, -[B]-, and -[B*]-; wherein A, B, and B* are distinct monomers; a homopolymer of monomer A is a highly conductive polymer; monomer B includes a reactive functional group selected to form a covalent link to a binding reagent; and monomer B* is monomer B covalently linked to the binding reagent. ### Claim 15 (depends on 14) The method of claim 14, wherein the copolymer has formula (I): -[A]x-[B]y-[B*]z- (I) wherein x, y, and z are the mole fractions of monomer A, B, and B* in the copolymer, respectively; and 0y+z. ### Claim 16 (depends on 14) The method of claim 14, wherein monomer A and monomer B, independently, are each an optionally substituted aniline monomer, an optionally substituted pyrrole monomer, or an optionally substituted thiophene monomer. ### Claim 17 (depends on 14) The method of claim 14, wherein monomer A is an EDOT monomer. ### Claim 18 (depends on 14) The method of claim 14, wherein monomer B is a 3-TE monomer or a TAA monomer. ### Claim 19 (depends on 1) The method of claim 1, wherein the binding reagent is a protein. ### Claim 20 (depends on 1) A method of detecting a plurality of analytes, comprising: contacting a sample suspected of containing at least one of the plurality of analytes with a sensor array including a plurality of sensor elements, wherein each sensor element includes a chemiresistive biosensor according to claim 1, and wherein each sensor element is configured to detect a different analyte. ### Claim 21 A method of making a sensor configured to detect an analyte, comprising: providing a high specific surface area substrate; coating a conductive polymer conformally on the high specific surface area substrate; and covalently linking a binding reagent to the conductive polymer, wherein the binding reagent has a specific affinity for the analyte. ### Claim 22 (depends on 21) The method of claim 21, wherein coating includes contacting the substrate with a vapor including an oxidant, a first monomer A, and a distinct second monomer B; wherein a homopolymer of monomer A is a highly conductive polymer; and monomer B includes a reactive functional group selected to form a covalent link to a binding reagent. ### Claim 23 (depends on 22) The method of claim 22, wherein covalently linking includes contacting the conductive polymer with the binding reagent and, optionally, a crosslinking reagent, thereby forming one or more covalent bonds covalently linking a binding reagent to the reactive functional group. ### Claim 24 (depends on 23) The method of claim 23, wherein, after covalently linking, the copolymer has formula (I): -[A]x-[B]y-[B*]z- (I) wherein monomer B* is monomer B covalently linked to the binding reagent; wherein x, y, and z are the mole fractions of monomer A, B, and B* in the copolymer, respectively; and 0y+z. ### Claim 25 (depends on 24) The method of claim 24, wherein monomer A and monomer B, independently, are each an optionally substituted aniline monomer, an optionally substituted pyrrole monomer, or an optionally substituted thiophene monomer. ### Claim 26 (depends on 24) The method of claim 24, wherein monomer A is an EDOT monomer. ### Claim 27 (depends on 24) The method of claim 24, wherein monomer B is a 3-TE monomer or a TAA monomer. ### Claim 28 (depends on 21) The method of claim 21, wherein the substrate includes an electro-spun polymer fiber mat. ### Claim 29 (depends on 21) The method of claim 21, wherein the substrate has a BET surface area of at least 5 m2/g. ### Claim 30 (depends on 21) The method of claim 21, wherein the binding reagent is a protein.