# US 2014/0193911 A1 · Thermoresponsive cell culture supports > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0193911 A1 - Document ID: US20140193911A1 - Status: Published application - Kind: A1 - Application number: 14/110,465 - Filed: 2012-04-06 - Published: 2014-07-10 - Earliest claimed: 2011-04-08 - Assignees: The University of Akron - Inventors: Bi-Min Newby; Nikul Patel; John Cavicchia; Ge Zhang - Web page: https://patentgrep.com/patent/US20140193911A1 ## CPC - C12N 5/0068: {General culture methods using substrates (for specific animal cell type C12N5/06)} - C12N 5/0662: {Stem cells} - C12N 5/0663: {Bone marrow mesenchymal stem cells (BM-MSC)} - C12N 5/0664: {Dental pulp stem cells, Dental follicle stem cells} - C12N 5/0665: {Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood} - C12N 5/0666: {Mesenchymal stem cells from hair follicles} - C12N 5/0667: {Adipose-derived stem cells [ADSC]; Adipose stromal stem cells} - C12N 5/0668: {Mesenchymal stem cells from other natural sources} - C12N 2533/12: Glass - C12N 2539/10: Coating allowing for selective detachment of cells, e.g. thermoreactive coating - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract The present invention relates to a cell culture support comprising a substrate and a thermoresponsive polymeric blend layer, wherein the polymeric blend layer comprises at least one thermoresponsive polymer and at least one network forming adhesion promoter. The present invention further relates a method of making a cell culture complex comprising: providing a substrate; blending at least one thermoresponsive polymer and at least one network forming adhesion promoter to provide a polymeric blend; applying a thin film of said polymeric blend to the substrate to provide a polymeric blend layer on the substrate; curing the polymeric blend layer on the substrate to provide a cell culture support; and depositing cells onto said cell culture support, wherein the cells may optionally further comprise medium, to provide a cell culture complex. ## Claims ### Claim 1 A cell culture support comprising: a substrate and a polymeric blend layer bound to said substrate, wherein the polymeric blend layer comprises at least one thermoresponsive polymer and at least one network forming adhesion promoter. ### Claim 2 (depends on 1) The support of claim 1, wherein the substrate is selected from the group consisting of polymeric materials, glasses, ceramics, metals, metal oxides, hydrated metal oxides, and combinations thereof. ### Claim 3 (depends on 1) The support of claim 1, wherein the at least one thermoresponsive polymer is selected from the group consisting of poly (N-isopropylacrylamide) (PNIPAAm), poly(N,N-diethylacrylamide) (PDEAAm), poly(N-vinlycaprolactam) (PVCL), poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA), and poly(ethylene oxide) (PEO), and combinations thereof. ### Claim 4 (depends on 1) The support of claim 1, wherein the at least one thermoresponsive polymer is poly (N-isopropylacrylamide) represented by the formula: ### Claim 5 (depends on 1) The support of claim 1, wherein the at least one network forming adhesion promoter is characterized as having functional amino or carboxylic acid groups. ### Claim 6 (depends on 1) The support of claim 1, wherein the at least one network forming adhesion promoter is an aminosilane. ### Claim 7 (depends on 1) The support of claim 1, wherein the at least one network forming adhesion promoter is selected from the group consisting of 3-aminopropyltriethoxysilane (APTES), 3-aminopropyldiethoxymethylsilane (APDEMS), and 3-aminopropyltrimethoxysilane (APTMS), and combinations thereof. ### Claim 8 (depends on 1) The support of claim 1, wherein the at least one network forming adhesion promoter is 3-aminopropyltriethoxysilane (APTES) represented by the formula: ### Claim 9 (depends on 1) The support of claim 1, wherein the polymer blend is characterized as having a thermoresponsive polymer to network forming adhesion promoter ratio (TRP:NFAP) of from about 90:10 to about 40:60. ### Claim 10 (depends on 1) The support of claim 1, wherein polymeric blend layer is devoid of adhesive proteins. ### Claim 11 (depends on 1) The support of claim 1, wherein the polymeric blend layer is devoid of plasma or e-beam treatment. ### Claim 12 A method of making a cell culture complex comprising: providing a substrate; blending at least one thermoresponsive polymer and at least one network forming adhesion promoter to provide a polymeric blend; applying a thin film of said polymeric blend to the substrate to provide a polymeric blend layer on the substrate; curing the polymeric blend layer on the substrate to provide a cell culture support; and depositing cells onto said cell culture support, wherein the cells may optionally further comprise medium, to provide a cell culture complex. ### Claim 13 (depends on 12) The method of claim 12, wherein the cultured cell layer comprises cells further characterized as anchor dependent cells. ### Claim 14 (depends on 12) The method of claim 12, wherein the cultured cell layer comprises cells further characterized as adhesive cells. ### Claim 15 (depends on 12) The method of claim 12, wherein the cultured cell layer comprises cells selected from the group consisting of fibroblasts, myoblasts, myotube cells, corneal cells, vascular endothelial cells, smooth muscle cells, cardiomyocytes, dermal cells, epidermal cells, mucosal epithelial cells, mesenchymal stem cells, ES cells, iPS cells, osteoblasts, osteocytes, chondrocytes, fat cells, neurons, hair root cells, dental pulp stem cells, β-cells, hepatocytes, and combinations thereof. ### Claim 16 (depends on 12) The method of claim 12, wherein the cultured cell layer comprises cells and medium. ### Claim 17 (depends on 12) The method of claim 12, wherein the thin film of said polymeric blend is spin-coated onto the substrate. ### Claim 18 (depends on 12) The method of claim 12, wherein polymeric blend layer is devoid of adhesive proteins. ### Claim 19 (depends on 12) The method of claim 12, wherein the polymeric blend layer is devoid of plasma or e-beam treatment. ### Claim 20 (depends on 12) The method of claim 12, wherein the detachment of the cultured cell layer is devoid of proteolytic enzymes or mechanical agitations.