# US 2014/0193901 A1 · Large scale cell harvesting method for pack-bed culture device

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- Number: US 2014/0193901 A1
- Document ID: US20140193901A1
- Status: Published application
- Kind: A1
- Application number: 14/148,631
- Filed: 2014-01-06
- Published: 2014-07-10
- Earliest claimed: 2013-01-07
- Assignees: CESCO Bioengineering Co., Ltd.
- Applicants: Cesco Bioengineering Co., Ltd.
- Inventors: Vincent LEE; Peter CHEN; Audrey CHEN; Alex LIU; Chungnan FU; Gray WANG; Hugo CHEN; King-Ming CHANG; Lewis HO
- Web page: https://patentgrep.com/patent/US20140193901A1

## CPC

- C12M 25/14: {Scaffolds; Matrices (in general C12N5/0068)}
- C12M 25/18: {Fixed or packed bed}
- C12M 47/02: {Separating microorganisms from the culture medium; Concentration of biomass (separating microorganisms from their culture media C12N1/02)}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

A large scale cell harvesting method for a pack-bed culture device by providing cell growing space that is loosely packed with a plurality of porous matrixes and/or matrix means. When a cell detaching step at least comprising shaking or spinning the culture chamber is performed, the unoccupied space is large enough for the porous matrixes collide to each other or to the culture chamber to generate momentum whereby the cells detach from the porous matrixes to harvest the cells.

## Claims

### Claim 1

A large scale cell harvesting method, comprising:
providing a pack-bed culture device configured with a culture chamber having a cell growing space defined within the culture chamber, wherein
the cell growing space is loosely packed with a plurality of porous matrixes,
a plurality of cells grow on the porous matrixes, and
a void volume is unoccupied space defined by the porous matrixes in the cell growing space;
performing a cell detaching step at least comprising shaking or spinning the culture chamber, wherein the void volume is large enough for the porous matrixes collide to each other or to the culture chamber to generate momentum whereby the cells detach from the porous matrixes; and
harvesting the cells.

### Claim 2 (depends on 1)

The method as claimed in claim 1, wherein the void volume is more than 25% v/v of the cell growing space.

### Claim 3 (depends on 1)

The method as claimed in claim 1, wherein the void volume is more than 33% v/v of the cell growing space.

### Claim 4 (depends on 1)

The method as claimed in claim 1, wherein the porous matrixes are provided with pore size from 50 um to 200 um and with porosity larger than 70%.

### Claim 5 (depends on 1)

The method as claimed in claim 1, wherein the cells are animal cells.

### Claim 6 (depends on 1)

The method as claimed in claim 1, wherein the cells are mammalian cells.

### Claim 7 (depends on 1)

The method as claimed in claim 1, wherein the cell detaching step further comprising enzyme digestion, and the enzyme comprises trypsin or EDTA.

### Claim 8 (depends on 1)

The method as claimed in claim 1, wherein the cell detaching step further comprising shaking with liquid or spinning with liquid.

### Claim 9 (depends on 8)

The method as claimed in claim 8, wherein the spinning with liquid is rotated clockwise and counter-clockwise intermittently.

### Claim 10 (depends on 1)

The method as claimed in claim 1, wherein the cell growing space is greater than 500 ml.

### Claim 11

A large scale cell harvesting method, comprising:
providing a pack-bed culture device configured with a culture chamber having a cell growing space defined within the culture chamber, wherein
the cell growing space is loosely packed with a plurality of matrix means, wherein the matrix means are porous and have porous matrixes therein,
a plurality of cells grow on the porous matrixes, and
a void volume is unoccupied space defined by the matrix means; in the cell growing space;
performing a cell detaching step at least comprising shaking or spinning the culture chamber, wherein the void volume is large enough for the matrix means collide to each other or to the culture chamber or flush by liquid to generate momentum whereby the cells detach from the porous matrixes; and
harvesting the cells.

### Claim 12 (depends on 11)

The method as claimed in claim 11, wherein the void volume is more than 10% v/v of the cell growing space.

### Claim 13 (depends on 10)

The method as claimed in claim 10, wherein the void volume is more than 33% v/v of the cell growing space.

### Claim 14 (depends on 10)

The method as claimed in claim 10, wherein the cells are animal cells.

### Claim 15 (depends on 11)

The method as claimed in claim 11, wherein the cells are mammalian cells.

### Claim 16 (depends on 11)

The method as claimed in claim 11, wherein the cell detaching step further comprising enzyme digestion, and the enzyme comprises trypsin or EDTA.

### Claim 17 (depends on 11)

The method as claimed in claim 11, wherein the cell detaching step further comprising shaking with liquid or spinning with liquid.

### Claim 18 (depends on 17)

The method as claimed in claim 17, wherein the spinning with liquid is rotated clockwise and counter-clockwise intermittently.

### Claim 19 (depends on 11)

The method as claimed in claim 11, wherein the matrix means have a ball shape, a cylinder shape, a rod shape or an oval shape.

### Claim 20 (depends on 11)

The method as claimed in claim 11, wherein the cell growing space is greater than 500 ml.