# US 2014/0193897 A1 · Bioreactor container and integrity check method for bioreactor containers > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0193897 A1 - Document ID: US20140193897A1 - Status: Published application - Kind: A1 - Application number: 14/130,061 - Filed: 2012-06-15 - Published: 2014-07-10 - Earliest claimed: 2011-06-30 - Assignees: Sartorius Stedim Biotech GmbH - Inventors: Martin Dahlberg; Isabelle Gay; Lars Boettcher; Stefan Obermann; Rainer Sandrock - Web page: https://patentgrep.com/patent/US20140193897A1 ## CPC - C12M 23/14: {Bags} - A61J 1/10: Bag-type containers - B32B 1/00: Layered products having a non-planar shape - B32B 3/30: characterised by a layer formed with recesses or projections, e.g. {hollows, grooves, protuberances, ribs (apertured layer B32B3/266; layer with cavities or internal voids B32B3/26)} - B32B 5/022: {Non-woven fabric} - B32B 5/024: {Woven fabric} - B32B 5/18: characterised by features of a layer {of} foamed material - C12M 23/02: {Form or structure of the vessel (large containers B65D88/00)} - C12M 23/26: {flexible (flexible containers for laboratory use B01L3/505)} - G01M 3/04: by detecting the presence of fluid at the leakage point - G01M 3/22: for pipes, cables or tubes; for pipe joints or seals; for valves; {for welds; for containers, e.g. radiators} - G01M 3/3218: {for flexible or elastic containers} - G01M 3/3272: {for verifying the internal pressure of closed containers} - A61J 1/16: Holders for containers - B32B 2307/726: Permeability to liquids, absorption - B32B 2307/7265: Non-permeable - F04C 2270/0421: Controlled or regulated - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} - A61J: CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE - B32B: LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM - G01M: TESTING STATIC OR DYNAMIC BALANCE OF MACHINES OR STRUCTURES; TESTING OF STRUCTURES OR APPARATUS, NOT OTHERWISE PROVIDED FOR - F04C: ROTARY-PISTON, OR OSCILLATING-PISTON, POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS (engines F03C); ROTARY-PISTON, OR OSCILLATING-PISTON, POSITIVE-DISPLACEMENT PUMPS ## Abstract A bioreactor container comprising has an at least locally flexible wall and at least one container opening. The wall of the bioreactor container has a fluid-tight inner sheet, and an at least locally fluid-permeable or structured outer sheet. A method for testing the integrity of the bioreactor container also is provided. ## Claims ### Claim 1 A bioreactor container (1) comprising: an at least locally flexible wall (3); at least one container opening (9a, 9b, 9c, 9d), wherein the wall (3) of the bioreactor container (1) has a fluid-tight inner sheet (5), and wherein the wall (3) of the bioreactor container (1) has an at least locally fluid-permeable outer sheet (7). ### Claim 2 (depends on 1) The bioreactor container (1) of claim 1, wherein the fluid-permeable outer sheet (7) comprises at least one of a woven textile, a nonwoven textile and a foam material. ### Claim 3 (depends on 1) The bioreactor container (1) of claim 1, wherein the fluid-permeable outer sheet (7) has a direction-dependent fluid permeability. ### Claim 4 A bioreactor container (1) comprising: an at least locally flexible wall (3); at least one container opening (9a, 9b, 9c, 9d), wherein the wall (3) of the bioreactor container (1) has a fluid-tight inner sheet (5), and wherein the wall (3) of the bioreactor container (1) has an at least locally structured outer sheet (7). ### Claim 5 (depends on 4) The bioreactor container (1) of claim 4, wherein the structured outer sheet (7) has depressions which are at least about 200 μm deep. ### Claim 6 (depends on 5) The bioreactor container (1) of claim 5, wherein the structured outer sheet (7) has elevations that are at most about 200 μm wide. ### Claim 7 (depends on 6) The bioreactor container (1) of claim 6, wherein the elevations and depressions of the structured outer sheet (7) are oriented along a preferential direction V. ### Claim 8 (depends on 4) The bioreactor container (1) of claim 4, wherein the fluid-permeable outer sheet (7) or the structured outer sheet (5) is connected releasably or nonreleasably to the fluid-impermeable inner sheet (5), and wherein the outer sheet (7) is formed as a sterile barrier. ### Claim 9 (depends on 4) The bioreactor container (1) of claim 4, wherein the at least one container opening (9a, 9b, 9c, 9d) of the bioreactor container (1) is enclosed in a first state by the outer sheet (7), wherein the at least one container opening (9a, 9b, 9c, 9d) is freely accessible in a second state, and wherein the bioreactor container (1) can be converted from the first state to the second state by local tearing and removal of the outer sheet (7). ### Claim 10 (depends on 1) A method for testing the integrity of a bioreactor container (1), having the steps: providing the bioreactor container (1) of claim 1; providing a bioreactor apparatus having a bioreactor container compartment (25); arranging the bioreactor container (1) at least partially in the bioreactor container compartment (25); connecting the at least one container opening (9a, 9b, 9c, 9d) of the bioreactor container to a fluid source (27); filling the bioreactor container (1) with a fluid from the fluid source (27) in order to generate a predetermined positive pressure Pi in the bioreactor container (1) at a first time Ti, the outer sheet (7) of the bioreactor container (1) bearing at least locally on an inner wall of the bioreactor container compartment (25); and determining whether the bioreactor container (1) is sufficiently leaktight.