# US 2014/0193886 A1 · Surfactants that improve the cleaning of lipid-based stains treated with lipases > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2014/0193886 A1 - Document ID: US20140193886A1 - Status: Published application - Kind: A1 - Application number: 14/074,558 - Filed: 2013-11-07 - Published: 2014-07-10 - Earliest claimed: 2009-12-21 - Assignees: Danisco US Inc. - Applicants: Danisco US Inc. - Inventors: Christian Adams; Katherine D. Collier; Michael Jay Pepsin; Brian Schmidt - Web page: https://patentgrep.com/patent/US20140193886A1 ## CPC - C11D 3/38627: {containing lipase} - C11D 1/62: Quaternary ammonium compounds - C11D 1/662: {Carbohydrates or derivatives} - C11D 1/72: Ethers of polyoxyalkylene glycols - C11D 1/75: Amino oxides - C11D 1/886: {Ampholytes containing P} - C11D 1/92: Sulfobetaines {; Sulfitobetaines} - C11D 3/221: {Mono, di- or trisaccharides or derivatives thereof} - C12N 9/20: Triglyceride splitting, e.g. by means of lipase - C12Y 301/01003: Triacylglycerol lipase (3.1.1.3) - C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C12Y: ENZYMES ## Abstract Described are compositions and methods relating to the removal of oily stains from fabrics and other surfaces using a lipase in combination with a selected surfactant to mediate the release of fatty acids generated by the lipase. The compositions and methods have application in, e.g., laundry cleaning and dishwashing. ## Claims ### Claim 1 A cleaning composition for removing oily stains, comprising: a) a lipolytic enzyme for hydrolyzing fatty acid esters present in the oily stain to produce free fatty acids, and b) a surfactant for solubilizing the free fatty acids in the cleaning composition, thereby releasing the free fatty acids from the stain, wherein the amount of release of fatty acids from the stain is greater than that achieved using an equivalent composition lacking the surfactant. ### Claim 2 (depends on 1) The cleaning composition of claim 1, wherein the cleaning composition is a laundry detergent or a dishwashing detergent. ### Claim 3 (depends on 1) The cleaning composition of claim 1, wherein the cleaning composition is a single composition comprising the lipolytic enzyme and the surfactant. ### Claim 4 (depends on 1) The cleaning composition of claim 1, wherein the cleaning composition is a two-part composition, the first part comprising the lipolytic enzyme and second part comprising the surfactant, wherein the first part and the second part are combined prior to contacting the stain. ### Claim 5 (depends on 1) The cleaning composition of claim 1, wherein the surfactant is a sugar-based non-ionic surfactant. ### Claim 6 (depends on 1) The cleaning composition of claim 1, wherein the surfactant is a maltopyranoside or a glucopyranoside. ### Claim 7 (depends on 1) The cleaning composition of claim 1, wherein the surfactant is a cyclic-maltopyranoside. ### Claim 8 (depends on 5) The cleaning composition of claim 5, wherein the sugar is maltose, glucose, or sucrose. ### Claim 9 (depends on 5) The cleaning composition of claim 5, wherein, the sugar-based surfactant has an aliphatic portion comprising at least 4 carbons. ### Claim 10 (depends on 1) The cleaning composition of claim 1, wherein the surfactant is selected from the group consisting of a Triton or oxide non-ionic surfactant, a zwitterionic surfactant, a FOS-choline or a sulfobetaine surfactant. ### Claim at position 11 11-13. (canceled) ### Claim 14 A method for removing an oily stain from a surface, comprising: contacting the surface with a lipolytic enzyme and a surfactant, hydrolyzing fatty acid esters present in the oily stain with the lipolytic enzyme to produce free fatty acids, and solubilizing the free fatty acids produced by the lipolytic enzyme with the surfactant, thereby removing the oily stain from the surface. ### Claim 15 (depends on 14) The method of claim 14, wherein the lipolytic enzyme and the surfactant are present in a single cleaning composition. ### Claim 16 (depends on 14) The method of claim 14, wherein the lipolytic enzyme and the surfactant are present in different cleaning compositions that are combined prior to the contacting. ### Claim 17 (depends on 14) The method of claim 14, wherein the lipolytic enzyme and the surfactant are present in different cleaning compositions that are combined upon the contacting. ### Claim 18 (depends on 14) The method of claim 14, wherein the method further includes rinsing the surface. ### Claim 19 (depends on 14) The method of claim 14, wherein the surfactant is a sugar-based non-ionic surfactant. ### Claim 20 (depends on 14) The method of claim 14, wherein the surfactant is a maltopyranoside, a glucopyranoside, or a cyclic-maltopyranoside. ### Claim 21 (depends on 19) The method of claim 19, wherein the sugar is maltose, glucose, or sucrose. ### Claim 22 (depends on 14) The method of claim 14, wherein the surfactant is selected from the group consisting of a Triton or oxide non-ionic surfactant, a zwitterionic surfactant, a FOS-choline or a sulfobetaine surfactant. ### Claim at position 21 23-24. (canceled) ### Claim 25 (depends on 14) The method of claim 14, wherein the surface is selected from the group consisting of a fabric surface, a dishware surface and a hard surface. ### Claim at position 23 26-27. (canceled)